Pfafferott C, Wenby R, Meiselman H J
Blood Cells. 1982;8(1):65-78.
The influence of cellular morphology on the rheologic behavior of human red blood cells (RBC) was examined for both fresh and ATP-depleted erythrocytes. Rheologic measurements included cone-plate viscometry, cell deformation by high-speed centrifugation and direct microscopic observation via a counter-rotating Rheoscope. Evaluation of the experimental observations indicated the importance of RBC shape as a determinant of RBC deformability such that either echinocytic or stomatocytic shape alterations resulted in decreased deformability. the influences of both intracellular viscosity and the ratio of intracellular to suspending medium viscosity on age-separated RBC rheologic behavior were also investigated via the rheoscope. Deformation at a given stress level increased with increasing media viscosity; at constant media viscosity and shear stress, young cells deformed more than old. However, identical cellular deformation could be achieved by comparing RBC at equal ratios of internal to external viscosity. The above mentioned results appear relevant to measurements of SS RBC mechanical behavior when dealing with either non-biconcave cells (e.g., ISC or low oxygen tension) or with cells having altered internal viscosity (e.g., ISC, increased MCHC, gel formation). The relative importance of these effects is, however, as yet unknown.
研究了细胞形态对新鲜和ATP耗竭的人红细胞（RBC）流变行为的影响。流变学测量包括锥板粘度测定、通过高速离心使细胞变形以及通过反向旋转流变仪进行直接显微镜观察。对实验观察结果的评估表明，红细胞形状作为红细胞变形性的决定因素很重要，因此棘形或口形的形状改变都会导致变形性降低。还通过流变仪研究了细胞内粘度以及细胞内粘度与悬浮介质粘度之比对按年龄分离的红细胞流变行为的影响。在给定应力水平下的变形随着介质粘度的增加而增加；在介质粘度和剪切应力恒定的情况下，年轻细胞比年老细胞变形更多。然而，通过比较内部与外部粘度相等的红细胞比例，可以实现相同的细胞变形。上述结果似乎与处理非双凹细胞（例如，缺铁性贫血或低氧张力）或内部粘度改变的细胞（例如，缺铁性贫血、平均血红蛋白浓度增加、凝胶形成）时对镰状细胞机械行为的测量有关。然而，这些影响的相对重要性尚不清楚。
