Xu Xiang-rong, Lin Hu, Zhang Xiao-xin, Li Jing-yi, Zhang Wei, Sun Wen-jun, Pan Yong-miao
Women's Hospital, School of Medicine, Zhejiang University Bioelectromagnetics Key Laboratory Zhejiang University, Hangzhou 310006, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2012 Mar;30(3):178-80.
To study the effects of 50-Hz extremely low frequency electromagnetic field (ELF-EMF) exposure on the pH of the adult male semen and the motoricity and motoricity parameters of spermatozoa.
Healthy adult male fresh semen was exposed to a 50-Hz EMF at 0.4 mT for 15, 30 and 60 min, respectively. The pH value of the semen, the motoricity and motoricity parameter of spermatozoa were detected and recorded in real time using the WLJY-9000 pattern chromatic color spermatozoa quality detection system.
Compared with parallel control group, the exposure of adult male fresh semen to a 50-Hz EMF at 0.4 mT for 15 min or 60 min could decrease significantly the motoricity (spermatozoa with a + b lever) and the activity ratio (spermatozoa with a + b + c lever)(P < 0.01). However, there were no significant differences of motoricity and the activity ratio between exposure group and control group (P > 0.05), and after exposure to a 50-Hz. EMF for 30 min the motoricity and the activity ratio of exposure group were inhibited, as compared with control group (P < 0.01 or P < 0.05). The pH value of the semen was not obvious changed (P > 0.05) when semen was exposed to a 50-Hz EMF of 0.4 mT for 15, 30 and 60 min.
In present experiment, it is suggested that the exposure of adult male fresh semen to a 50-Hz EMF in vitro could inhibit the motoricity and the activity ratio, but not affect the pH value of the semen within 60 min.
研究50赫兹极低频电磁场（ELF - EMF）暴露对成年男性精液pH值以及精子活力和活力参数的影响。
将健康成年男性新鲜精液分别暴露于0.4毫特斯拉的50赫兹电磁场中15、30和60分钟。使用WLJY - 9000型彩色精子质量检测系统实时检测并记录精液的pH值、精子活力和活力参数。
与平行对照组相比，成年男性新鲜精液暴露于0.4毫特斯拉的50赫兹电磁场中15分钟或60分钟可显著降低精子活力（a + b级精子）和活动率（a + b + c级精子）（P < 0.01）。然而，暴露组与对照组之间的活力和活动率无显著差异（P > 0.05），且暴露于50赫兹电磁场30分钟后，暴露组的活力和活动率与对照组相比受到抑制（P < 0.01或P < 0.05）。当精液暴露于0.4毫特斯拉的50赫兹电磁场中15、30和60分钟时，精液的pH值无明显变化（P > 0.05）。
在本实验中，提示成年男性新鲜精液体外暴露于50赫兹电磁场可抑制精子活力和活动率，但在60分钟内不影响精液的pH值。
