[单核巨噬细胞对煤焦油沥青提取物诱导人支气管上皮细胞恶性转化的影响]

[The effects of monocyte-macrophages on malignant transformation of human bronchial epithelial cells induced by extracts from coal tar pitch].

作者信息

Zhou Fan-jing, Zhang Shao-feng, Feng Fei-fei, Yan Zhen, Wang Wei, Wu Yi-ming

机构信息

College of Public Health, Zhengzhou University, Zhengzhou 450001, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2012 Apr;30(4):241-5.

PMID:22804928

Abstract

OBJECTIVE

To study the effects of monocyte-macrophages (THP-1) in malignant transformation of human bronchial epithelial cells (BEAS-2B) cells induced by coal tar pitch (CTP) and the expression of TNF-α in the process of the cell malignant transformation.

METHODS

BEAS-2B cells and THP-1 Cells were divided into four groups: coal tar pitch (CTP) group, benzo(a)pyrene [B(a)P] group, dimethyl sulfoxide (DMSO) group, BEAS-2B and THP-1 co-culture (co-culture group) group. Carcinogenesis model was established. The soft agar colony formation, chromosome aberrations and cell cycle tests were used to detect the cellular malignant transformation. The ELISA assay was utilized to measure the levels of TNF-α in the supernatant of CTP group and co-culture group.

RESULTS

The chromosome number abnormalities could be observed in early stage of the experiment (the 10th generation cells), which showed the increased ratio of aneuploid to polyploid, and the decreased number of diploid. The colony formation rate of co-culture group (the 20th generation cells) was 17.63‰ ± 0.97‰, which was significantly higher than that (13.94‰ ± 0.84‰) of CTP group and that (12.96‰ ± 1.62‰) of B(a)P group (P < 0.05). The proportion of S phase cells in the co-culture group was 44.49% ± 0.68%, which was significantly higher than that (38.19% ± 1.26%) of CTP group and that (36.41% ± 1.19%) of B(a)P group (P < 0.05). The TNF-α level in the co-culture group was significantly higher than that in CTP group (P = 0.001).

CONCLUSION

Monocyte-Macrophages can accelerate the malignant transformation of BEAS-2B cells induced by CTP and increase the expression level of TNF-α.

摘要

目的

研究单核巨噬细胞（THP-1）在煤焦油沥青（CTP）诱导人支气管上皮细胞（BEAS-2B）恶性转化中的作用及细胞恶性转化过程中肿瘤坏死因子-α（TNF-α）的表达情况。

方法

将BEAS-2B细胞和THP-1细胞分为四组：煤焦油沥青（CTP）组、苯并(a)芘[B(a)P]组、二甲基亚砜（DMSO）组、BEAS-2B与THP-1共培养（共培养组）组。建立致癌模型。采用软琼脂集落形成、染色体畸变及细胞周期检测等方法检测细胞恶性转化情况。利用酶联免疫吸附测定（ELISA）法检测CTP组和共培养组上清液中TNF-α水平。

结果

实验早期（第10代细胞）即可观察到染色体数目异常，表现为非整倍体与多倍体比例增加，二倍体数目减少。共培养组（第20代细胞）集落形成率为17.63‰±0.97‰，显著高于CTP组（13.94‰±0.84‰）和B(a)P组（12.96‰±1.62‰）（P<0.05）。共培养组S期细胞比例为44.49%±0.68%，显著高于CTP组（38.19%±1.26%）和B(a)P组（36.41%±1.19%）（P<0.05）。共培养组TNF-α水平显著高于CTP组（P=0.001）。