[Effect of human monocytes on coal tar pitch extract-induced TRAF2 mRNA expression in human bronchial epithelial cells].

OBJECTIVE

To explore the regulation mechanism of coal tar pitch smoke extracts( CTPE) on tumor necrosis factor receptor-associated factor 2( TRAF2) mRNA expression in co-cultured model of human monocytes( THP-1) and BEAS-2B cells.

METHODS

The co-cultured THP-1 and BEAS-2B cells were simulated by 3 μg/m L CTPE. The cells at passage 9 were treated with tumor necrosis factor-α( TNF-α) neutralization antibody and c-Jun N-terminal kinase( JNK) inhibitors SP600125, and continued toculture at passage 15( neutralizing antibody group) and established the BEAS-2B cells of same generation as control group. The expression of TRAF2 mRNA was detected by realtime fluorescent quantitative polymerase chain reaction.

RESULTS

The expression of TRAF2 mRNA and c-Jun mRNA in different groups of TNF-α neutralization antibody and JNK kinase inhibitors SP600125 groups were decreased compared with CC10, respectively the groups of 48h( 0. 19 ± 0. 02) and 36h( 0. 03 ± 0. 02) were lowest. The expression of TRAF2 mRNA in groups of TNF-α neutralization antibody and JNK kinase inhibitors SP600125 groups( respectively 1. 73 ± 0. 04 and 1. 88 ± 0. 05) were both significantly increased compared with control group( 1. 07 ± 0. 06), and lower than CC15( 2. 23 ±0. 09).

CONCLUSION

THP-1 could up-regulate the expression of TRAF2 mRNA through TNF-α in the BEAS-2B cells stimulated by CTPE, and there maybe negative feedback effect between TRAF2 and AP-1.