Wang Yong-xing, Yao Wu, Gao Bin, Yao Yong-cheng, Wu Yi-ming, Hao Chang-fu
Department of Occupational Health Collage of Public Health, Zhengzhou University, Zheng-zhou, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2012 May;30(5):326-30.
To explore the DNA methylation levels of genome in cFb transdifferentiation induced by SiO2 in rats.
The primary macrophages and fibrocytes of SD rats were co-cultured directly and indirectly, which were exposed to SiO2 at the doses of 25, 50 and 100 g/ml. The transdifferentiation of cFb was identified with immunohistochemical assay. The genomic DNA methylation levels of cFb were detected with HPLC.
Under the condition of indirect co-culture, as compared with control group, the genomic DNA methylation levels of cFb exposed to SiO2 at the doses of 25, 50 and 100 g/ml reduced by 19.9%, 26.9% and 30.3%, respectively (P < 0.05); as compared with cFb exposed to 100 g/ml SiO2, the genomic DNA methylation levels of cFb exposed to 5-aza-dC decreased by 22.0% (P < 0.05). Under the condition of ThinCert(TM) direct co-culture, as compared with control group, the genomic DNA methylation levels of cFb exposed to SiO2 at the doses of 25, 50 and 100 g/ml reduced by 22.2%, 30.2% and 36.7%, respectively (P < 0.05); as compared with cFb exposed to 100 g/ml SiO2, the genomic DNA methylation levels of cFb exposed to 5-aza-dC decreased by 20.6% (P < 0.05).
Under the co-culture condition in vitro, SiO2 could reduce the genomic DNA methylation levels of cFb. The ThinCert(TM) direct co-culture can be used to study the silicosis fibrosis.
探讨二氧化硅诱导大鼠肺成纤维细胞转分化过程中基因组DNA甲基化水平变化。
将SD大鼠原代巨噬细胞与成纤维细胞直接和间接共培养，分别给予25、50和100μg/ml二氧化硅染毒。采用免疫组织化学法鉴定肺成纤维细胞转分化情况，应用高效液相色谱法检测肺成纤维细胞基因组DNA甲基化水平。
间接共培养条件下，与对照组比较，25、50和100μg/ml二氧化硅染毒组肺成纤维细胞基因组DNA甲基化水平分别降低19.9%、26.9%和30.3%（P<0.05）；与100μg/ml二氧化硅染毒组比较，5-氮杂-2'-脱氧胞苷组肺成纤维细胞基因组DNA甲基化水平降低22.0%（P<0.05）。直接共培养条件下，与对照组比较，25、50和100μg/ml二氧化硅染毒组肺成纤维细胞基因组DNA甲基化水平分别降低22.2%、30.2%和36.7%（P<0.05）；与100μg/ml二氧化硅染毒组比较，5-氮杂-2'-脱氧胞苷组肺成纤维细胞基因组DNA甲基化水平降低20.6%（P<0.05）。
体外共培养条件下，二氧化硅可降低肺成纤维细胞基因组DNA甲基化水平。直接共培养可用于矽肺纤维化的研究。
