Zhang Mei-li, Ni Pei-hua, Cai Chang-ping, Chen Ni-jun, Wang Shi-li
Department of Otorhinolaryngology, Ruijin Hospital, Shanghai Jiaotong University School of Medcine, Shanghai 200025, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 Mar;47(3):212-7.
To investigate the relationship between the promoter polymorphism of IL-4 and IL-6 and chronic rhinosinusitis (CRS).
One hundred and twenty-three patients with CRS and 239 healthy controls in Shanghai region were chosen in this study. The genotype of IL-4 gene -33T>C and -590C>T were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and the genotype of IL-10 gene -1082A>G was determined using amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) method. Statistical calculations were performed using SAS 8.2 software.
Significant differences were found in genotype distribution of -33T>C and -590C>T between the CRS group and the control group (χ2=6.6013, P=0.0102, χ2=6.6013, P=0.0304), and -33T>C remained significant following application of the Bonferroni correction (P<0.025). The relative risks of CRS with -33T>C and -590C>T were 1.818(P=0.0236, 95%CI 1.084-3.050) and 1.838 (P=0.0147, 95%CI 1.127-2.997). There was linkage disequilibrium (LD) between the -33T>C and -590C>T. The coefficient of linkage disequilibrium (D') was 0.77 and the related coefficient (r2) was 0.54. The -33T/-590T haplotype was associated with CRS and the relative risk was 1.653 (P=0.0130, 95%CI 1.107-2.469). There were only two genotypes of IL-10 gene-1082A>G and the frequencies of the AA and AG genotypes were not different between the CRS and control groups.
The promoter polymorphism of IL-4 -33T>C and -590C>T were associated with the susceptibility of CRS and the -33T/-590T haplotype was a risk factor for CRS, but there were no association between the -1082A>G and CRS.
探讨白细胞介素-4（IL-4）和白细胞介素-6（IL-6）启动子多态性与慢性鼻-鼻窦炎（CRS）之间的关系。
本研究选取上海地区123例CRS患者和239名健康对照者。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测IL-4基因-33T>C和-590C>T的基因型，采用扩增阻滞突变系统-聚合酶链反应（ARMS-PCR）方法检测IL-10基因-1082A>G的基因型。使用SAS 8.2软件进行统计学计算。
CRS组与对照组之间-33T>C和-590C>T的基因型分布存在显著差异（χ2=6.6013，P=0.0102，χ2=6.6013，P=0.0304），应用Bonferroni校正后-33T>C仍具有显著性（P<0.025）。-33T>C和-590C>T患CRS的相对风险分别为1.818（P=0.0236，95%可信区间1.084-3.050）和1.838（P=0.0147，95%可信区间1.127-2.997）。-33T>C与-590C>T之间存在连锁不平衡（LD）。连锁不平衡系数（D'）为0.77，相关系数（r2）为0.54。-33T/-590T单倍型与CRS相关，相对风险为1.653（P=0.0130，95%可信区间1.107-2.469）。IL-10基因-1082A>G只有两种基因型，CRS组与对照组之间AA和AG基因型的频率无差异。
IL-4 -33T>C和-590C>T启动子多态性与CRS易感性相关，-33T/-590T单倍型是CRS的危险因素，但-1082A>G与CRS之间无关联。
