脊髓灰质炎病毒感染的HeLa细胞对胍敏感的钠离子积累
Guanidine-sensitive Na+ accumulation by poliovirus-infected HeLa cells.
作者信息
Nair C N, Stowers J W, Singfield B
出版信息
J Virol. 1979 Jul;31(1):184-9. doi: 10.1128/JVI.31.1.184-189.1979.
Abstract
The Na+ content of poliovirus-infected HeLa S3 cells increased during the late phase of virus replication, after virus inhibition of host cell protein synthesis and in coincidence with late viral functions. Guanidine hydrochloride blocked the rise in Na+ content, whereas the antiguanidine agent choline fully reversed the guanidine block. Expression of one or more late viral functions was essential for Na+ accumulation to occur because accumulation was inhibited by cycloheximide or guanidine added to the infected culture during the late phase. Increased adenosine triphosphatase activity appears to be primarily responsible for Na+ accumulation by virus-infected cells.
摘要
脊髓灰质炎病毒感染的HeLa S3细胞的Na⁺含量在病毒复制后期、病毒抑制宿主细胞蛋白质合成后以及与病毒晚期功能同时出现时增加。盐酸胍阻止了Na⁺含量的升高，而抗胍剂胆碱完全逆转了胍的阻断作用。一种或多种病毒晚期功能的表达对于Na⁺积累的发生至关重要，因为在后期向感染培养物中添加环己酰亚胺或胍会抑制积累。腺苷三磷酸酶活性的增加似乎是病毒感染细胞积累Na⁺的主要原因。
相似文献
1
Guanidine-sensitive Na+ accumulation by poliovirus-infected HeLa cells.脊髓灰质炎病毒感染的HeLa细胞对胍敏感的钠离子积累
J Virol. 1979 Jul;31(1):184-9. doi: 10.1128/JVI.31.1.184-189.1979.
2
Guanidine uptake by HeLa cells and its inhibition by some antiguanidine agents.HeLa细胞对胍的摄取及其受某些抗胍剂的抑制作用。
J Gen Virol. 1987 Nov;68 ( Pt 11):2889-97. doi: 10.1099/0022-1317-68-11-2889.
3
Modification of membrane permeability in poliovirus-infected HeLa cells: effect of guanidine.
J Gen Virol. 1983 Apr;64 (Pt 4):787-93. doi: 10.1099/0022-1317-64-4-787.
4
Dependence of guanidine sensitivity of poliovirus replication on the concentration of monovalent cations in the culture medium.脊髓灰质炎病毒复制对胍敏感性的依赖性与培养基中单价阳离子浓度的关系
J Gen Virol. 1987 Apr;68 ( Pt 4):1195-8. doi: 10.1099/0022-1317-68-4-1195.
5
The P2 and P3 regions of the poliovirus genome are preferentially translated at alkaline pH in infected HeLa cells.脊髓灰质炎病毒基因组的P2和P3区域在感染的HeLa细胞中于碱性pH条件下优先进行翻译。
J Gen Virol. 1988 Mar;69 ( Pt 3):583-90. doi: 10.1099/0022-1317-69-3-583.
6
Guanidine inhibition of poliovirus growth. Partial elimination by protease antagonists and low temperature.胍对脊髓灰质炎病毒生长的抑制作用。蛋白酶拮抗剂和低温可部分消除这种抑制作用。
Can J Microbiol. 1974 Jun;20(6):817-24. doi: 10.1139/m74-126.
7
The mechanism of guanidine inhibition of poliovirus growth in vitro.胍抑制脊髓灰质炎病毒体外生长的机制。
Life Sci II. 1970 Dec 8;9(23):1351-8. doi: 10.1016/0024-3205(70)90116-5.
8
Complete replication of poliovirus in vitro: preinitiation RNA replication complexes require soluble cellular factors for the synthesis of VPg-linked RNA.脊髓灰质炎病毒在体外的完全复制：起始前RNA复制复合体合成与VPg相连的RNA需要可溶性细胞因子。
J Virol. 1995 Sep;69(9):5516-27. doi: 10.1128/JVI.69.9.5516-5527.1995.
9
Inhibition of host cell protein synthesis by UV-inactivated poliovirus.紫外线灭活脊髓灰质炎病毒对宿主细胞蛋白质合成的抑制作用。
J Virol. 1977 Jan;21(1):259-67. doi: 10.1128/JVI.21.1.259-267.1977.
10
Poliovirus coat protein as the site of guanidine action.脊髓灰质炎病毒衣壳蛋白作为胍作用的位点。
Virology. 1977 Aug;81(1):25-36. doi: 10.1016/0042-6822(77)90055-1.
引用本文的文献
1
Modification of membrane permeability by animal viruses.动物病毒对膜通透性的改变。
Adv Virus Res. 1995;45:61-112. doi: 10.1016/s0065-3527(08)60058-5.
2
Myxoviruses do not induce non-specific alterations in membrane permeability early on in infection.黏液病毒在感染初期不会引起膜通透性的非特异性改变。
Arch Virol. 1983;77(2-4):139-53. doi: 10.1007/BF01309263.
3
Poliovirus-induced alterations in HeLa cell membrane functions.脊髓灰质炎病毒引起的海拉细胞膜功能改变。
J Virol. 1982 Nov;44(2):445-9. doi: 10.1128/JVI.44.2.445-449.1982.
4
Reversion by hypotonic medium of the shutoff of protein synthesis induced by encephalomyocarditis virus.低渗介质对脑心肌炎病毒诱导的蛋白质合成阻断的逆转作用。
J Virol. 1981 Feb;37(2):535-40. doi: 10.1128/JVI.37.2.535-540.1981.
5
Monovalent cation metabolism and cytopathic effects of poliovirus-infected HeLa cells.脊髓灰质炎病毒感染的HeLa细胞的单价阳离子代谢及细胞病变效应
J Virol. 1981 Jan;37(1):268-73. doi: 10.1128/JVI.37.1.268-273.1981.
6
Regulation of protein synthesis in virus-infected animal cells.病毒感染动物细胞中蛋白质合成的调控。
Adv Virus Res. 1986;31:229-92. doi: 10.1016/s0065-3527(08)60265-1.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A CYTOPLASMIC STRUCTURE INVOLVED IN THE SYNTHESIS AND ASSEMBLY OF POLIOVIRUS COMPONENTS.一种参与脊髓灰质炎病毒成分合成与组装的细胞质结构。
J Mol Biol. 1964 Apr;8:541-55. doi: 10.1016/s0022-2836(64)80010-3.
3
SPECIFIC INHIBITION OF REPLICATION OF ANIMAL VIRUSES.动物病毒复制的特异性抑制
Science. 1963 Oct 4;142(3588):24-33. doi: 10.1126/science.142.3588.24.
4
Poliovirus-induced RNA polymerase and the effects of virus-specific inhibitors on its production.脊髓灰质炎病毒诱导的RNA聚合酶及其病毒特异性抑制剂对其产生的影响。
Proc Natl Acad Sci U S A. 1963 Jun;49(6):843-9. doi: 10.1073/pnas.49.6.843.
5
In vitro guanidino-resistance and guanidino-dependence of poliovirus.脊髓灰质炎病毒的体外胍抗性和胍依赖性
Experientia. 1962 Nov 15;18:518-9. doi: 10.1007/BF02151608.
6
Antiviral effect of guanidine.胍的抗病毒作用。
Science. 1961 Aug 25;134(3478):558-9. doi: 10.1126/science.134.3478.558.
7
Selective and reversible inhibition of initiation of protein synthesis in mammalian cells.哺乳动物细胞中蛋白质合成起始的选择性和可逆性抑制。
J Mol Biol. 1974 May 15;85(2):195-211. doi: 10.1016/0022-2836(74)90360-x.
8
Blocking of guanidine action on poliovirus multiplication.胍对脊髓灰质炎病毒增殖作用的阻断
Virology. 1971 Sep;45(3):653-63. doi: 10.1016/0042-6822(71)90179-6.
9
Comparison of polysomal structures of uninfected and poliovirus infected HeLa cells.未感染和脊髓灰质炎病毒感染的HeLa细胞多核糖体结构的比较。
Virology. 1971 May;44(2):239-48. doi: 10.1016/0042-6822(71)90256-x.
10
The role of cytoplasmic membranes in poliovirus biosynthesis.细胞质膜在脊髓灰质炎病毒生物合成中的作用。
Virology. 1970 Sep;42(1):100-11. doi: 10.1016/0042-6822(70)90242-4.
Zotero 插件