Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
Asian Pac J Trop Med. 2012 Sep;5(9):686-91. doi: 10.1016/S1995-7645(12)60107-2.
To evaluate nephroprotective potential of Solanum xanthocarpum (S. xanthocarpum) fruit extract(SXE) against gentamicin (GM) induced nephrotoxicity and renal dysfunction.
Twenty-four Wistar rats were divided into four groups (n=6). Control rats that received normal saline (i.p.) and 0.5% carboxymethyl cellulose (p.o.) per day for 8 d. Nephrotoxicity was induced in rats by intraperitoneal administration of GM (100 mg/kg/d for 8 d) and were treated with SXE (200 and 400 mg/kg/d (p.o.) for 8 d). Plasma and urine urea and creatinine, kidney weight, urine output, blood urea nitrogen, renal enzymatic and non-enzymatic antioxidants and lipid peroxidation was evaluated along with histopathological investigation in various experimental groups of rats.
It was observed that the GM treatment induced significant elevation (P<0.001) in plasma and urine urea, creatinine, kidney weight, blood urea nitrogen, renal lipid peroxidation along with significant decrement (P<0.001) in urine output, renal enzymatic and non-enzymatic antioxidants. SXE 200 and 400 mg/kg treatment to GM treated rats recorded significant decrement (up to P<0.001) in plasma and urine urea and creatinine, renal lipid peroxidation along with significant increment (up to P<0.001) in renal enzymatic and non-enzymatic antioxidants. Histological observations of kidney tissues too correlated with the biochemical observations.
These finding powerfully supports that S. xanthocarpum fruit extract acts in the kidney as a potent scavenger of free radicals to prevent the toxic effects of GM both in the biochemical and histopathological parameters and thus validates its ethnomedicinal use.
评估茄科茄（S. xanthocarpum）果实提取物（SXE）对庆大霉素（GM）诱导的肾毒性和肾功能障碍的肾保护潜力。
将 24 只 Wistar 大鼠分为四组（n=6）。对照组大鼠每天接受生理盐水（腹腔注射）和 0.5%羧甲基纤维素（口服）8 天。通过腹腔注射 GM（每天 100mg/kg，连续 8 天）诱导大鼠肾毒性，并给予 SXE（200 和 400mg/kg/d（口服），连续 8 天）治疗。评估了各组大鼠的血浆和尿液尿素和肌酐、肾脏重量、尿量、血尿素氮、肾酶和非酶抗氧化剂以及脂质过氧化，并进行了组织病理学检查。
GM 处理导致血浆和尿液尿素、肌酐、肾脏重量、血尿素氮、肾脂质过氧化显著升高（P<0.001），同时尿量、肾酶和非酶抗氧化剂显著降低（P<0.001）。SXE 200 和 400mg/kg 治疗 GM 处理大鼠，血浆和尿液尿素和肌酐、肾脂质过氧化显著降低（高达 P<0.001），同时肾酶和非酶抗氧化剂显著升高（高达 P<0.001）。肾组织的组织学观察也与生化观察相关。
这些发现有力地支持茄科茄果实提取物在肾脏中作为自由基的有效清除剂，可防止 GM 在生化和组织病理学参数方面的毒性作用，从而验证了其民族医学用途。
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