Department of Biomedical Sciences and Public Health, Section of Pathologic Anatomy and Histopathology, Polytechnic University of the Marche/United Hospitals, Torrette (Ancona), Italy.
Oral Dis. 2013 Jan;19(1):92-9. doi: 10.1111/j.1601-0825.2012.01961.x. Epub 2012 Jul 13.
Radicular cysts occur as a result of the immunological response to continuous antigenic stimulation from root canals. We correlated the immunophenotypical composition of the lymphoid infiltrate to the microvessel density expressed by the count of CD34 reactive endothelial cells in radicular cysts.
Thirty-four cases of radicular cysts were evaluated by immunohistochemistry, using antibodies against B- and T-cell antigens (CD20, CD3, CD4, CD8) and against the endothelial cell marker CD34. Statistical analysis was performed.
In the epithelium, we observed a low amount of lymphoid infiltrate in all 34 radicular cysts, and a strong significant negative correlation between T and B lymphocytes and between T-helper and T-cytotoxic/suppressor lymphocytes. In the cyst capsule, we observed a significant positive correlation between B and T lymphocytes, B and T-cytotoxic/suppressor lymphocytes, T and T-helper lymphocytes and between the number of CD34+ blood vessels and T and T-helper lymphocytes, respectively. We observed a statistically significant correlation between percentage of CD34+ vessels and inflammatory infiltrate grade.
Both humoral and cellular immune reactions and neovascularization are likely to occur in the complex events of tissue destruction. The inflammatory infiltrate has an important role in neoangiogenesis and consequently in radicular cysts development and growth.
根囊肿是由于根管内持续的抗原刺激引起的免疫反应而发生的。我们将淋巴浸润的免疫表型组成与 CD34 反应性内皮细胞计数所表示的根囊肿中的微血管密度相关联。
通过免疫组织化学，使用针对 B 和 T 细胞抗原（CD20、CD3、CD4、CD8）和内皮细胞标记物 CD34 的抗体，评估了 34 例根囊肿病例。进行了统计分析。
在囊肿上皮中，我们观察到所有 34 例根囊肿中淋巴浸润的数量很少，T 和 B 淋巴细胞之间以及 T-helper 和 T-细胞毒性/抑制性淋巴细胞之间存在强烈的显著负相关。在囊肿囊壁中，我们观察到 B 和 T 淋巴细胞之间、B 和 T-细胞毒性/抑制性淋巴细胞之间、T 和 T-helper 淋巴细胞之间以及 CD34+血管数量与 T 和 T-helper 淋巴细胞之间存在显著的正相关。我们观察到 CD34+血管百分比与炎症浸润程度之间存在统计学显著相关性。
体液和细胞免疫反应以及新血管生成可能发生在组织破坏的复杂事件中。炎症浸润在新血管生成中具有重要作用，因此在根囊肿的发展和生长中起重要作用。
