检测和定量分析人炎症性根尖囊肿和肉芽肿中的肥大细胞、血管内皮生长因子和微血管密度。
Detection and quantification of mast cell, vascular endothelial growth factor, and microvessel density in human inflammatory periapical cysts and granulomas.
机构信息
Department of Dentistry, State University of Montes Claros, Montes Claros, MG, Brazil.
出版信息
Int Endod J. 2012 Sep;45(9):859-64. doi: 10.1111/j.1365-2591.2012.02043.x. Epub 2012 Apr 5.
AIM
To identify and quantify mast cell (MC), vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in human periapical cysts and granulomas.
METHODOLOGY
Archived samples of cysts (n = 40) and granulomas (n = 28) were sectioned and stained with toluidine blue. MCs were identified and counted. Immunohistochemical reactions were employed to evaluate the tissue expression of VEGF and vessels. MVD was estimated by determining the areas of tissue labelled with CD31 antibody. The data were analysed using the Mann-Whitney test (P < 0.05).
RESULTS
MCs were observed in the peripheral regions of both lesion types, whilst VEGF and MVD were distributed in the stroma. The presence of MCs was higher in cysts than in granulomas (P < 0.05). VEGF and MVD expression were similar in these lesions.
CONCLUSIONS
The highest number of MCs was observed in cysts. Moreover, the identification of VEGF and MVD was consistent with the immune mechanisms involved in the lesions.
目的
鉴定和量化人根尖囊肿和肉芽肿中的肥大细胞（MC）、血管内皮生长因子（VEGF）的表达和微血管密度（MVD）。
方法
对存档的囊肿（n=40）和肉芽肿（n=28）样本进行切片，并用甲苯胺蓝染色。鉴定并计数 MC。采用免疫组织化学反应评估 VEGF 和血管的组织表达。通过确定用 CD31 抗体标记的组织区域来估计 MVD。使用曼-惠特尼检验（P＜0.05）对数据进行分析。
结果
MC 存在于两种病变类型的周边区域，而 VEGF 和 MVD 分布在基质中。囊肿中 MC 的存在高于肉芽肿（P＜0.05）。这些病变中 VEGF 和 MVD 的表达相似。
结论
在囊肿中观察到的 MC 数量最多。此外，VEGF 和 MVD 的鉴定与涉及病变的免疫机制一致。
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