建立一种用于诊断伯克霍尔德氏菌感染的诊断系统。

Development of a diagnostic system for Burkholderia pseudomallei infections.

机构信息

Division of Microbiology, Defence Research and Development Establishment, Jhansi Road, Gwalior, 474 002, India.

出版信息

World J Microbiol Biotechnol. 2012 Jul;28(7):2465-71. doi: 10.1007/s11274-012-1053-y. Epub 2012 May 23.

Abstract

Monoclonal antibodies were generated against whole cell lysate of Burkholderia pseudomallei. Two out of 6 monoclonal antibodies were found specific and exhibited high affinity against B. pseudomallei, one of which, was utilized to develop sandwich ELISA for detection of specific B. pseudomallei antigen. Immunoassays were found to be specific as no reaction was observed with closely related Burkholderia and Pseudomonas species. Blood samples from experimentally infected mice were found positive for isolation till 4 days post infection (DPI) and ELISA till 10 DPI. One out of 40 sick animal serum samples tested in Thailand was found positive by sandwich ELISA that was earlier confirmed by isolation of B. pseudomallei. The results indicate the potentiality of the assay for its applicability in specific diagnosis of septicaemic melioidosis.

摘要

针对 Burkholderia pseudomallei 的全细胞裂解物产生了单克隆抗体。在 6 种单克隆抗体中，有 2 种被发现具有特异性，并对 B. pseudomallei 表现出高亲和力，其中一种被用于开发夹心 ELISA 以检测特异性 B. pseudomallei 抗原。免疫测定被发现是特异性的，因为与密切相关的 Burkholderia 和 Pseudomonas 物种没有观察到反应。从实验感染的小鼠血液样本中发现，在感染后 4 天（DPI）内可通过分离法检测到，在 10 DPI 内可通过 ELISA 检测到。在泰国检测的 40 份患病动物血清样本中，有 1 份通过夹心 ELISA 检测呈阳性，该结果之前通过分离 B. pseudomallei 得到了证实。这些结果表明该检测方法具有潜在的应用价值，可用于败血症性类鼻疽的特异性诊断。