IgG酶联免疫吸附测定、斑点免疫测定及间接血凝试验对败血症型类鼻疽诊断价值的回顾性研究

Retrospective study on the diagnostic value of IgG ELISA, dot immunoassay and indirect hemagglutination in septicemic melioidosis.

作者信息

Wongratanacheewi S, Sermswan R W, Anuntagool N, Sirisinha S

机构信息

Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand.

出版信息

Asian Pac J Allergy Immunol. 2001 Jun;19(2):129-33.

PMID:11699719

Abstract

Three serological methods for diagnosis of melioidosis were compared with the culture method currently used as the "gold standard". The diagnostic values of the serological methods were evaluated retrospectively in 306 patients residing in an endemic area. The enzyme-linked immunosorbent assay (ELISA), using affinity purified antigen for detecting specific IgG antibody, showed a slightly higher specificity (86.0%) than the dot immunoassay (DOT) (84.0%) and both were superior to indirect hemagglutination (IHA) (72.0%). The sensitivity of DOT (96.4%) and ELISA (85.7%) were considerably higher than that of IHA (50.0%). The primary benefit of the high negative predictive value of both ELISA (96.4%) and DOT (99.0%) in an area of high prevalence is the ability to rule out most of the non-melioidosis patients.

摘要

将三种用于诊断类鼻疽的血清学方法与目前作为“金标准”使用的培养方法进行了比较。在306名居住在流行地区的患者中对血清学方法的诊断价值进行了回顾性评估。使用亲和纯化抗原检测特异性IgG抗体的酶联免疫吸附测定（ELISA）显示出比斑点免疫测定（DOT）（84.0%）略高的特异性（86.0%），且两者均优于间接血凝试验（IHA）（72.0%）。DOT（96.4%）和ELISA（85.7%）的敏感性显著高于IHA（50.0%）。在高流行地区，ELISA（96.4%）和DOT（99.0%）两者的高阴性预测值的主要益处在于能够排除大多数非类鼻疽患者。