Maranhão R C, Roland I A, Hirata M H
Heart Institute of the São Paulo University Medical School Hospital, (Instituto do Caração HC-FMUSP), Brazil.
Lipids. 1990 Nov;25(11):701-5. doi: 10.1007/BF02544037.
The influence of lipolytic mechanisms on the transfer of phospholipids and unesterified cholesterol from artificial emulsions, serving as chylomicron models to other plasma lipoproteins, mainly high density lipoproteins (HDL) were tested in vivo. The emulsions labeled with radioactive lipids were injected into the bloodstream of rats (controls) and the results were compared with those obtained from rats that had previously been treated with Triton WR 1339 or heparin. Plasma clearance and the distribution of cholesteryl esters, phospholipids and unesterified cholesterol in the different plasma lipoprotein fractions were then determined. Whereas virtually no cholesteryl esters were found in d greater than 1.006 g/mL density fraction of the three experimental groups, 2.8 +/- 1.3% of the injected phospholipids were in the 1.063-1.210 g/L density fraction of the Triton treated rats, and 12.6 +/- 5.4% of the heparin treated rats, as compared to 10.1 +/- 1.7% in controls. This indicates that lipolysis directly influences phospholipid transfer to HDL. In contrast, free-cholesterol transfer to HDL, besides being less pronounced than phospholipid transfer, was enhanced by Triton and diminished by heparin, indicating that lipolytic mechanisms were not important determinants in this process.
在体内测试了脂解机制对磷脂和未酯化胆固醇从作为乳糜微粒模型的人工乳液向其他血浆脂蛋白（主要是高密度脂蛋白，HDL）转移的影响。将用放射性脂质标记的乳液注入大鼠（对照组）的血液中，并将结果与先前用Triton WR 1339或肝素处理过的大鼠的结果进行比较。然后测定血浆清除率以及不同血浆脂蛋白组分中胆固醇酯、磷脂和未酯化胆固醇的分布。在三个实验组中，密度大于1.006 g/mL的组分中几乎未发现胆固醇酯，而在Triton处理的大鼠中，注入的磷脂有2.8±1.3%存在于密度为1.063 - 1.210 g/L的组分中，肝素处理的大鼠中为12.6±5.4%，而对照组为10.1±1.7%。这表明脂解直接影响磷脂向HDL的转移。相反，游离胆固醇向HDL的转移，除了不如磷脂转移明显外，Triton可增强其转移，肝素则使其减弱，这表明脂解机制在该过程中并非重要的决定因素。
