Cunha G M M, Silva V M A, Bessa K D G, Bitencourt M A O, Macêdo U B O, Freire-Neto F P, Martins R R, Assis C F, Lemos T M A M, Almeida M G, Freire A C G
Centro de Ciências da Saúde, Faculdade de Farmácia, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte, Rua Gal. Gustavo Cordeiro de Farias, s/n, 2º Andar, Petrópolis, CEP: 59012-570, Natal, Rio Grande do Norte, Brazil.
Acta Parasitol. 2012 Jun;57(2):160-6. doi: 10.2478/s11686-012-0026-5. Epub 2012 May 13.
Schistosomiasis is caused by Schistosoma mansoni and is a public health problem in Brazil. The typical granulomatous lesion is associated with the increase in the oxidative damage by generation of free radicals. The aim of this work was to correlate some oxidative stress markers with the worm burden on carriers of schistosomiasis (n = 30) in the acute phase in comparison to healthy subjects (n = 30). The pro-oxidant parameter used was the colorimetric quantification of reactive substances to thiobarbituric acid, while the antioxidant markers used were blood content of reduced glutathione and determination of the activity of catalase. The worm burden was assessed by Kato-Katz method. The results pointed out that initially there was no difference in the catalase activity. However, there was a positive correlation between the increase in parasitic load and intensity of lipid peroxidation, and decrease in the content of reduced glutathione. Additionally, only the aspartate aminotransferase levels presented to be high, while there was a decrease in bilirubin level. Therefore, a possible association between the establishment of the oxidative stress in tissue and the parasitic load of Schistosoma mansoni is suggested.
血吸虫病由曼氏血吸虫引起，是巴西的一个公共卫生问题。典型的肉芽肿病变与自由基产生导致的氧化损伤增加有关。这项工作的目的是将一些氧化应激标志物与急性期血吸虫病携带者（n = 30）的虫负荷相关联，并与健康受试者（n = 30）进行比较。所使用的促氧化参数是对硫代巴比妥酸反应性物质的比色定量，而所使用的抗氧化标志物是还原型谷胱甘肽的血液含量和过氧化氢酶活性的测定。通过加藤厚涂片法评估虫负荷。结果指出，最初过氧化氢酶活性没有差异。然而，寄生虫负荷的增加与脂质过氧化强度之间存在正相关，且还原型谷胱甘肽含量降低。此外，只有天冬氨酸转氨酶水平较高，而胆红素水平降低。因此，提示组织中氧化应激的建立与曼氏血吸虫的寄生虫负荷之间可能存在关联。
