Samadieh Hadi, Mohammadi Gholam-Reza, Maleki Mohsen, Borji Hassan, Azizzadeh Mohammad, Heidarpour Mohammad
Dept. of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Dept. of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Iran J Parasitol. 2017 Jan-Mar;12(1):46-55.
The aim of this study was to investigate the role of oxidative stress in the pathology of ovine dicrocoeliasis.
During Dec 2013 - Oct 2014, seventy-two sheep (1-3 years) with liver dicrocoeliasis along with 47 healthy sheep were selected from animals admitted for slaughtering at slaughterhouse located in Neyshabour, Razavi Khorasan Province, Iran.
In comparison to healthy control, the malondialdehyde (MDA) level and serum total antioxidant capacity were significantly higher in the parasitized group (<0.05). A significant increase in liver MDA concentration (<0.05) of parasitized group was also observed. Packed cell volume (PCV), zinc, iron, total bilirubin and albumin sera levels were significantly lower in the parasitized group (<0.05). In parasitized sheep, a significant positive correlation was seen between serum MDA concentration and the activity of aspartate aminotransferase (AST). On the other hand, the concentration of serum MDA was inversely correlated with the value of PCV. No significant differences were observed for MDA concentration and total antioxidant capacity between normal and abnormal hepatic lobes in the parasitized animals. Oxidative stress markers (MDA and total antioxidant capacity in serum and liver samples) showed no significant correlations with the extent of pathological lesions and serum variables of liver injury in the parasitized sheep. No significant correlation was observed between oxidative stress markers and the fluke's number in the parasitized animals.
Oxidative stress may play an important role in the erythrocyte destruction in sheep naturally infected with However, no clear relationships were observed between the oxidative stress, hepatic damage and parasite burden.
本研究旨在探讨氧化应激在绵羊双腔吸虫病病理学中的作用。
2013年12月至2014年10月期间，从伊朗拉扎维霍拉桑省尼沙布尔一家屠宰场接收待屠宰的动物中，选取了72只患有肝双腔吸虫病的绵羊（1 - 3岁）以及47只健康绵羊。
与健康对照组相比，感染寄生虫组的丙二醛（MDA）水平和血清总抗氧化能力显著更高（<0.05）。还观察到感染寄生虫组肝脏MDA浓度显著升高（<0.05）。感染寄生虫组的红细胞压积（PCV）、锌、铁、血清总胆红素和白蛋白水平显著更低（<0.05）。在感染寄生虫的绵羊中，血清MDA浓度与天冬氨酸转氨酶（AST）活性之间存在显著正相关。另一方面，血清MDA浓度与PCV值呈负相关。在感染寄生虫的动物中，正常和异常肝叶之间的MDA浓度和总抗氧化能力未观察到显著差异。氧化应激标志物（血清和肝脏样本中的MDA和总抗氧化能力）与感染寄生虫绵羊的病理损伤程度和肝脏损伤血清变量之间未显示出显著相关性。在感染寄生虫的动物中，氧化应激标志物与吸虫数量之间未观察到显著相关性。
氧化应激可能在自然感染双腔吸虫的绵羊红细胞破坏中起重要作用。然而，氧化应激、肝脏损伤和寄生虫负荷之间未观察到明确的关系。
