Department of Biotechnology, Heritage Institute of Technology, Chowbaga Road, Anandapur, Kolkata, 700107, India.
Phytother Res. 2013 May;27(5):725-30. doi: 10.1002/ptr.4780. Epub 2012 Jul 17.
Disaccharide intolerance is the inability to digest certain carbohydrates due to a lack of one or more intestinal disaccharidases (e.g., lactase, maltase, isomaltase and sucrase). Symptoms include diarrhea, abdominal distention and flatulence. Management of the disorder by external enzymes supplementation has not yet been attempted. We report that the medicinal plant Tinospora cordifolia contains substantial amounts of all disaccharidases required for intestinal digestion of carbohydrates. The plant is also a rich source of saccharifying amylase. We recovered (units/100 g fresh stem) amylase: 49,000+500, maltase: 400+50, isomaltase: 130+50, sucrase: 4500+500, acid lactase: 350+30, cellobiase: 35+10 and trehalase: 40+10 by buffer extraction of the blended stem. Crude enzymes in the forms of stem powder, lyophilized aqueous extract and ethanol precipitated protein were found to be stable. Disaccharidases were optimally active at 50 (0) C in the pH range of 4-5. Lactase was an acid lactase similar to the type linked with human lactose intolerance. Enzymes were catalytically stable in the pH range of 2-7 and temperature range of up to 40 (0) C. T. cordifolia enzyme was non-toxic up to a dose of 200 mg protein/kg body weight.
双糖不耐受是指由于缺乏一种或多种肠道二糖酶（如乳糖酶、麦芽糖酶、异麦芽糖酶和蔗糖酶）而无法消化某些碳水化合物。症状包括腹泻、腹胀和胀气。目前尚未尝试通过外源性酶补充来治疗这种疾病。我们报告称，药用植物三叶鬼针草含有大量的碳水化合物肠道消化所需的所有二糖酶。该植物也是糖化淀粉酶的丰富来源。我们通过混合茎的缓冲液提取回收了（单位/100g 新鲜茎）：淀粉酶：49000+500、麦芽糖酶：400+50、异麦芽糖酶：130+50、蔗糖酶：4500+500、酸性乳糖酶：350+30、纤维二糖酶：35+10 和海藻糖酶：40+10。茎粉、冻干水提物和乙醇沉淀蛋白等形式的粗酶均稳定。二糖酶在 50（0）C、pH 值为 4-5 的条件下具有最佳活性。乳糖酶是一种酸性乳糖酶，类似于与人类乳糖不耐受相关的类型。酶在 pH 值为 2-7 和温度高达 40（0）C 的范围内具有催化稳定性。T. cordifolia 酶的蛋白毒性剂量高达 200mg/kg 体重。
