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环 AMP 依赖蛋白激酶调节亚基与环 CMP 琼脂糖的结合。

Binding of regulatory subunits of cyclic AMP-dependent protein kinase to cyclic CMP agarose.

机构信息

Institute of Pharmacology, Hannover Medical School, Hannover, Germany.

出版信息

PLoS One. 2012;7(7):e39848. doi: 10.1371/journal.pone.0039848. Epub 2012 Jul 9.

DOI:10.1371/journal.pone.0039848
PMID:22808067
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3392273/
Abstract

The bacterial adenylyl cyclase toxins CyaA from Bordetella pertussis and edema factor from Bacillus anthracis as well as soluble guanylyl cyclase α(1)β(1) synthesize the cyclic pyrimidine nucleotide cCMP. These data raise the question to which effector proteins cCMP binds. Recently, we reported that cCMP activates the regulatory subunits RIα and RIIα of cAMP-dependent protein kinase. In this study, we used two cCMP agarose matrices as novel tools in combination with immunoblotting and mass spectrometry to identify cCMP-binding proteins. In agreement with our functional data, RIα and RIIα were identified as cCMP-binding proteins. These data corroborate the notion that cAMP-dependent protein kinase may serve as a cCMP target.

摘要

百日咳博德特氏菌的细菌腺苷酸环化酶毒素 CyaA 和炭疽芽孢杆菌的水肿因子以及可溶性鸟苷酸环化酶 α(1)β(1)合成环嘧啶核苷酸 cCMP。这些数据提出了 cCMP 结合的效应蛋白的问题。最近，我们报道 cCMP 激活 cAMP 依赖性蛋白激酶的调节亚基 RIα 和 RIIα。在这项研究中，我们使用了两种 cCMP 琼脂糖基质作为新工具，结合免疫印迹和质谱法来鉴定 cCMP 结合蛋白。与我们的功能数据一致，RIα 和 RIIα 被鉴定为 cCMP 结合蛋白。这些数据证实了 cAMP 依赖性蛋白激酶可能作为 cCMP 靶标的观点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e8b/3392273/db0e0f644d96/pone.0039848.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e8b/3392273/2d652dd0cd2a/pone.0039848.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e8b/3392273/29377433f486/pone.0039848.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e8b/3392273/143942e2c2cb/pone.0039848.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e8b/3392273/1c472e951c57/pone.0039848.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e8b/3392273/db0e0f644d96/pone.0039848.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e8b/3392273/2d652dd0cd2a/pone.0039848.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e8b/3392273/29377433f486/pone.0039848.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e8b/3392273/143942e2c2cb/pone.0039848.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e8b/3392273/1c472e951c57/pone.0039848.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e8b/3392273/db0e0f644d96/pone.0039848.g005.jpg

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2
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3
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