Department of Epidemiology, Capital Institute of Pediatrics, Beijing, China.
PLoS One. 2012;7(7):e40606. doi: 10.1371/journal.pone.0040606. Epub 2012 Jul 11.
The SNP (rs2230500) in PRKCH (the gene encoding protein kinase C η) is associated with ischemic stroke and cerebral hemorrhage in the Chinese population, but the molecular mechanisms are not clear. The aim of the present study is to investigate the association between the SNP and atherosclerosis that is common pathological basis of ischemic stroke and cerebral hemorrhage.
METHODOLOGY/PRINCIPAL FINDINGS: We examined the associations of the SNP with carotid intima-media thickness (CIMT), atherosclerosis diagnosed by CIMT, and factors related with inflammation in the Beijing Child Blood Pressure Study. A total of 1190 subjects participated in the follow-up study. The SNP was genotyped by allele-specific real-time PCR assay. The SNP (rs2230500) in PRKCH was significantly associated with CIMT (in far wall of left common carotid arteries, P = 0.016; in far wall of right common carotid arteries, P = 0.012) under a recessive model after adjustment for age, gender, smoking, and hypertension. The SNP was also significantly associated with complement C3 (P = 0.012) under a dominant model after adjustment for age, gender, and high sensitivity C-reactive protein.
CONCLUSIONS/SIGNIFICANCE: Our data provide evidence that the SNP (rs2230500) in PRKCH decreases the risk of CIMT that is a worthwhile predictor of stroke and complement system possibly mediates this process.
SNP(rs2230500)位于 PRKCH（蛋白激酶 C η 基因），与中国人群的缺血性卒中和脑出血有关，但分子机制尚不清楚。本研究旨在探讨 SNP 与动脉粥样硬化的关系，动脉粥样硬化是缺血性卒中和脑出血的共同病理基础。
方法/主要发现：我们通过颈动脉内膜中层厚度（CIMT）检查，研究 SNP 与动脉粥样硬化的关系，CIMT 用于诊断动脉粥样硬化，以及与炎症相关的因素与北京儿童血压研究有关。共有 1190 名受试者参加了随访研究。采用等位基因特异性实时 PCR 检测 SNP。PRKCH 中的 SNP(rs2230500)在调整年龄、性别、吸烟和高血压后，在隐性模型下与 CIMT（左颈总动脉远壁，P=0.016；右颈总动脉远壁，P=0.012）显著相关。调整年龄、性别和高敏 C 反应蛋白后，SNP 也在显性模型下与补体 C3（P=0.012）显著相关。
结论/意义：我们的数据提供了证据，表明 PRKCH 中的 SNP(rs2230500)降低了 CIMT 的风险，CIMT 是中风的一个有价值的预测指标，补体系统可能介导了这一过程。
