Department of Nursing, Yuanpei University, Hsinchu, Taiwan.
Am J Chin Med. 2012;40(4):845-59. doi: 10.1142/S0192415X12500632.
Lavender essential oil (LEO) is one the most favorite and widely used essential oils in aromatherapy. Many studies have demonstrated its functions in calming, assisting sleep, reducing pain and muscular spasms and its antiseptic function. To date, however, the mechanism of LEO on inflammation response is not well understood. In this study, we examined the effect of LEO on 5 μg/ml lipopolysaccharide (LPS) induced inflammation reaction in human monocyte THP-1 cells. We found treatment of 0.1% LEO significantly increased cell viability and inhibited the IL-1β and superoxide anion generation in LPS-stimulated THP-1 cells. Treatment with LEO down-regulated both LPS-induced protein levels of phospho-NF-κB and membrane Toll-like receptor 4. To determine whether the chaperone protein was involved in the reaction, we determined the levels of Heat Shock Protein 70 (HSP70). Our results showed that LEO increased HSP70 expression in LPS-stimulated THP-1 cells, suggesting that the LEO inhibited LPS-induced inflammatory effect might be associated with the expression of HSP70.
薰衣草精油（LEO）是芳疗中最受欢迎和广泛使用的精油之一。许多研究已经证明了它在镇静、辅助睡眠、减轻疼痛和肌肉痉挛以及其防腐功能方面的作用。然而，迄今为止，LEO 对炎症反应的机制还不是很清楚。在这项研究中，我们研究了 LEO 对人单核细胞 THP-1 细胞中 5μg/ml 脂多糖（LPS）诱导的炎症反应的影响。我们发现，0.1%的 LEO 处理显著增加了细胞活力，并抑制了 LPS 刺激的 THP-1 细胞中白细胞介素-1β（IL-1β）和超氧阴离子的生成。LEO 处理还下调了 LPS 诱导的磷酸化 NF-κB 和膜 Toll 样受体 4 的蛋白水平。为了确定伴侣蛋白是否参与了反应，我们测定了热休克蛋白 70（HSP70）的水平。结果表明，LEO 增加了 LPS 刺激的 THP-1 细胞中 HSP70 的表达，提示 LEO 抑制 LPS 诱导的炎症作用可能与 HSP70 的表达有关。
