Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong RD, Shijiazhuang 050021, China.
Hebei Province Engineering Laboratory for Plant Breeding and Germplasm Enhancement of Stress-Tolerant Plants, 286 Huaizhong RD, Shijiazhuang 050021, China.
New Phytol. 2012 Oct;196(1):125-138. doi: 10.1111/j.1469-8137.2012.04236.x. Epub 2012 Jul 18.
• Under cadmium (Cd) stress, Solanum nigrum accumulated threefold more Cd in its leaves and was tolerant to Cd, whereas its low Cd-accumulating relative, Solanum torvum, suffered reduced growth and marked oxidative damage. However, the physiological mechanisms that are responsible for differential Cd accumulation and tolerance between the two Solanum species are largely unknown. • Here, the involvement of antioxidative capacity and the accumulation of organic and amino acids in response to Cd stress in the two Solanum species were assessed. • Solanum nigrum contains higher antioxidative capacity than does S. torvum under Cd toxicity. Metabolomics analysis indicated that Cd treatment also markedly increased the production of several organic and amino acids in S. nigrum. Pretreatment with proline and histidine increased Cd accumulation; moreover, pretreatment with citric acid increased Cd accumulation in leaves but decreased Cd accumulation in roots, which indicates that its biosynthesis could be linked to Cd long-distance transport and accumulation in leaves. • Our data provide novel metabolite evidence regarding the enhancement of citric acid and amino acid biosynthesis in Cd-treated S. nigrum, support the role of these metabolites in improving Cd tolerance and accumulation, and may help to provide a better understanding of stress adaptation in other Solanum species.
• 在镉（Cd）胁迫下，龙葵在叶片中积累了三倍的 Cd，并且对 Cd 具有耐受性，而其低 Cd 积累的相对物，龙葵，则生长受到抑制，并出现明显的氧化损伤。然而，导致这两个茄属物种在 Cd 积累和耐受性方面存在差异的生理机制在很大程度上尚不清楚。• 在这里，评估了抗氧化能力的参与以及对两种茄属植物 Cd 胁迫的有机和氨基酸的积累。• 在 Cd 毒性下，龙葵比龙葵具有更高的抗氧化能力。代谢组学分析表明，Cd 处理还显著增加了龙葵中几种有机和氨基酸的产生。脯氨酸和组氨酸预处理增加了 Cd 的积累；此外，柠檬酸预处理增加了叶片中 Cd 的积累，但减少了根部 Cd 的积累，这表明其生物合成可能与 Cd 的长距离运输和在叶片中的积累有关。• 我们的数据提供了关于 Cd 处理的龙葵中柠檬酸和氨基酸生物合成增强的新代谢物证据，支持这些代谢物在提高 Cd 耐受性和积累方面的作用，并可能有助于更好地理解其他茄属物种的应激适应。
