Mi Zhaorong, Liu Pinlin, Du Lin, Han Tao, Wang Chao, Fan Xifeng, Liu Huichao, He Songlin, Wu Juying
School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China.
Henan Provincial Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement, Xinxiang 453003, China.
Plants (Basel). 2023 Oct 29;12(21):3713. doi: 10.3390/plants12213713.
The relationship between metabolite changes and biological endpoints in response to cadmium (Cd) stress remains unclear. Fountain grass has good Cd enrichment and tolerance abilities and is widely used in agriculture and landscaping. We analyzed the metabolic responses by detecting the metabolites through UPLC-MS and examined the relationships between metabolite changes and the characteristics of morphology and physiology to different Cd stress in fountain grass. Our results showed that under Cd stress, 102 differential metabolites in roots and 48 differential metabolites in leaves were detected, with 20 shared metabolites. Under Cd stress, most of the carbohydrates in leaves and roots decreased, which contributed to the lowered leaf/root length and fresh weight. In comparison, most of the differential amino acids and lipids decreased in the leaves but increased in the roots. Almost all the differential amino acids in the roots were negatively correlated with root length and root fresh weight, while they were positively correlated with malondialdehyde content. However, most of the differential amino acids in the leaves were positively correlated with leaf length and leaf fresh weight but negatively correlated with malondialdehyde content. Metabolic pathway analysis showed that Cd significantly affects seven and eight metabolic pathways in the leaves and roots, respectively, with only purine metabolism co-existing in the roots and leaves. Our study is the first statement on metabolic responses to Cd stress and the relationships between differential metabolites and biological endpoints in fountain grass. The coordination between various metabolic pathways in fountain grass enables plants to adapt to Cd stress. This study provides a comprehensive framework by explaining the metabolic plasticity and Cd tolerance mechanisms of plants.
镉（Cd）胁迫下代谢物变化与生物学终点之间的关系尚不清楚。喷泉草具有良好的镉富集和耐受能力，广泛应用于农业和园林绿化。我们通过超高效液相色谱-质谱联用仪检测代谢物来分析代谢响应，并研究了喷泉草对不同镉胁迫下代谢物变化与形态和生理特征之间的关系。我们的结果表明，在镉胁迫下，根部检测到102种差异代谢物，叶片检测到48种差异代谢物，其中有20种共有代谢物。在镉胁迫下，叶片和根部的大多数碳水化合物含量下降，这导致叶/根长度和鲜重降低。相比之下，叶片中大多数差异氨基酸和脂质含量下降，而根部则增加。根部几乎所有差异氨基酸与根长和根鲜重呈负相关，而与丙二醛含量呈正相关。然而，叶片中大多数差异氨基酸与叶长和叶鲜重呈正相关，但与丙二醛含量呈负相关。代谢途径分析表明，镉分别显著影响叶片和根部的7条和8条代谢途径，只有嘌呤代谢在根和叶中同时存在。我们的研究首次阐述了喷泉草对镉胁迫的代谢响应以及差异代谢物与生物学终点之间的关系。喷泉草中各种代谢途径之间的协调使植物能够适应镉胁迫。本研究通过解释植物的代谢可塑性和镉耐受机制提供了一个全面的框架。
