Chongqing Key Laboratory of Forage & Herbivorce, College of Animal Science and Technology, Southwest University, Chongqing 400716, People's Republic of China.
Br J Nutr. 2013 Mar 14;109(5):867-72. doi: 10.1017/S0007114512002681. Epub 2012 Jul 19.
The present study was conducted to determine the adjuvant effect of arginine in mice immunised with inactivated vaccine. Mice immunised with an inactivated Pasteurella multocida vaccine and fed diets supplemented with 0·2 % (vaccine-0·2 %) or 0·5 % (vaccine-0·5 %) arginine exhibited 100 % protection from a challenge with P. multocida serotype A (CQ2) at a dose of 4·4 × 105 colony-forming units (2LD50; median lethal dose), when compared with mice receiving no arginine supplementation. Meanwhile, antibody titres in the vaccine-0·2 % arginine group were much higher than those in the vaccine-oil adjuvant group before challenge and at 36 h post-infection. Furthermore, immunisation with the inactivated vaccine and dietary supplementation with 0·2 % arginine increased serum levels of glutathione peroxidase, in comparison with immunisation with the inactivated vaccine and an oil adjuvant. Collectively, dietary arginine supplementation confers an immunostimulatory effect in mice immunised with the inactivated P. multocida vaccine. The present results also indicate that optimal supplemental doses of arginine are 0·2-0·5 % in the mouse model.
本研究旨在确定精氨酸对已用灭活疫苗免疫的小鼠的佐剂效应。用灭活多杀性巴氏杆菌疫苗免疫并饲喂添加 0.2%（疫苗-0.2%）或 0.5%（疫苗-0.5%）精氨酸的饮食的小鼠，在接受 4.4×105 个集落形成单位（2LD50；半数致死剂量）的 A 型多杀性巴氏杆菌（CQ2）攻毒时，具有 100%的保护作用，而未接受精氨酸补充的小鼠则没有这种作用。同时，在攻毒前和感染后 36 小时，疫苗-0.2%精氨酸组的抗体滴度明显高于疫苗-油佐剂组。此外，与用灭活疫苗和油佐剂免疫相比，用灭活疫苗和 0.2%精氨酸饮食补充免疫可提高血清谷胱甘肽过氧化物酶水平。总之，日粮精氨酸补充可对已用灭活多杀性巴氏杆菌疫苗免疫的小鼠产生免疫刺激作用。本研究结果还表明，在小鼠模型中，精氨酸的最佳补充剂量为 0.2%-0.5%。
