College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou, PR China.
Colloids Surf B Biointerfaces. 2013 Jan 1;101:177-82. doi: 10.1016/j.colsurfb.2012.06.010. Epub 2012 Jun 19.
A new electrochemical hemoglobin (Hb) biosensor was constructed based on a Fe(3)O(4) mesoporous spheres modified carbon ionic liquid electrode (CILE), which showed excellent electrocatalytic ability towards the reduction of trichloroacetic acid. CILE was prepared by using N-hexylpyridinium hexafluorophosphate (HPPF(6)) as the modifier and the binder in the carbon paste. Ultraviolet-visible and Fourier transform infrared spectroscopic results indicated that Hb molecules retained the native structure in the chitosan and Fe(3)O(4) mesoporous spheres composite film. Electrochemical results indicated that a pair of well-defined redox peaks appeared in 0.1 mol/L phosphate buffer solution (PBS) with the formed potential (E(0')) as -0.287 V (vs. SCE), indicating that the direct electron transfer of Hb in the composite film was realized. Electrochemical behaviors of Hb were carefully investigated with the electrochemical parameters such as electron transfer coefficient (α), electron transfer number (n), and heterogeneous electron transfer rate constant (k(s)) calculated. Due to the specific characteristics of Fe(3)O(4) mesoporous spheres present on the electrode surface, the electron transfer rate of Hb was greatly promoted. The Hb modified electrode exhibited excellent electrocatalytic properties with wider linear range and lower detection limit.
一种基于 Fe(3)O(4)介孔球修饰的碳离子液体电极（CILE）的新型电化学血红蛋白（Hb）生物传感器被构建，该传感器对三氯乙酸的还原具有出色的电催化能力。CILE 通过使用 N-己基吡啶六氟磷酸盐（HPPF(6)）作为修饰剂和碳糊中的粘合剂来制备。紫外-可见和傅里叶变换红外光谱结果表明，Hb 分子在壳聚糖和 Fe(3)O(4)介孔球复合膜中保留了天然结构。电化学结果表明，在形成的电位（E(0'))为-0.287 V（相对于 SCE）的 0.1 mol/L 磷酸盐缓冲溶液（PBS）中出现了一对定义良好的氧化还原峰，表明 Hb 在复合膜中实现了直接电子转移。仔细研究了 Hb 的电化学行为，并计算了电子转移系数（α）、电子转移数（n）和异相电子转移速率常数（k(s)）等电化学参数。由于电极表面存在 Fe(3)O(4)介孔球的特殊特性，Hb 的电子转移速率大大提高。修饰后的 Hb 电极具有出色的电催化性能，具有更宽的线性范围和更低的检测限。
