Department of Biology, Faculty of Sciences, Alzahra University, Tehran, Iran.
J Environ Radioact. 2012 Nov;113:171-6. doi: 10.1016/j.jenvrad.2012.04.009. Epub 2012 Jul 17.
A new isolate, Kocuria sp. ASB 107 from the Ab-e-Siah mineral radioactive spring (Ramsar, Mazandaran Province, Iran) was characterized on the basis of morphological and biochemical characteristics plus 16S rRNA gene sequencing. The isolate is most closely related to Kocuria rosea DSM 20447(T) (99.7% sequence similarity) and Kocuria polaris DSM 14382(T) (99.5%). This strain has some resistance to various genotoxic stresses, such as ionizing radiation, ultraviolet (256 nm- UV) and corona discharge. The 90% lethal doses (D(10)) for gamma-rays and 256 nm-UV are 2 kGy and 400 J m(-2), respectively, in definite cell concentration. Moreover, the resistance for a definite energy of corona discharge is 10 s, about 10 times greater than that of Escherichia coli. The growth temperature of the strain ASB 107 is 0-37 °C in TSB (tryptic soy broth). This study is the first report on the psychrotrophic radio-resistant bacteria belonging to the Kocuria genus isolated from Ab-e-Siah spring.
从伊朗拉什特省马赞达兰省的 Ab-e-Siah 矿泉放射性泉水中分离到一株新的节杆菌属菌株 ASB 107，该菌基于形态学和生物化学特征以及 16S rRNA 基因测序进行了鉴定。该分离株与玫瑰色节杆菌 DSM 20447（T）（99.7%序列相似性）和极地节杆菌 DSM 14382（T）（99.5%序列相似性）最为密切相关。该菌株对各种遗传毒性应激具有一定的抗性，如电离辐射、紫外线（256nm-UV）和电晕放电。在一定细胞浓度下，γ射线和 256nm-UV 的 90%致死剂量（D（10））分别为 2kGy 和 400Jm（-2）。此外，电晕放电的抗性为 10s，约为大肠杆菌的 10 倍。菌株 ASB 107 的生长温度在 TSB（胰蛋白酶大豆肉汤）中为 0-37°C。这是首次报道从 Ab-e-Siah 泉水中分离到属于节杆菌属的耐辐射嗜冷菌。
