Najari Maryam, Moosavi-Nejad Zahra, Javaheri Elham Sadat Seyad Javad, Asgarani Ezat
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, 1993893973, Tehran, Iran.
Iran J Microbiol. 2017 Dec;9(6):356-362.
Because of importance of catalase in various industries, efforts have been made to find more suitable bacterial sources for catalase production. is one of well-known catalase-producing genus. This is the first report about a new catalase-overproducing bacterial strain, sp. ASB 107.
sp. ASB 107 had been isolated from Abe-Siah Spring in Ramsar in our previous report. The bacterial biomass freezed, thawed and then lysed by three different operations separately: ultrasound, lysing buffer and enzymatic digestion. The crude extract was subjected to ammonium sulfate precipitation (40 and 60% saturation). Quality and quantity of the semi-purification was checked by electrophoresis and measuring specific activity, respectively.
sp. ASB 107 can be lysed by a freeze-thaw stage followed by lysozyme digestion and not by lysing buffer and not by ultrasound. Surprisingly specific activity of catalase in crude extract from sp. ASB 107 was measured to be 195, 370 U/mg protein which is too much higher than other bacterial strains. The bacterium showed a relatively long growth curve about 40 hours. Semi-purification using ammonium sulfate precipitation was led in an increased specific activity up to about 7×10 U/mg protein implying more than 3.6-fold purification.
We have showed natural catalase-overproducing ability of sp. ASB 107. Yield and purity of catalase from sp. ASB 107 showed great potential in industrial application suggesting the strain as good source for mass production of catalase for treatment of HO-containing wastewater in comparison to other bacterial sources.
由于过氧化氢酶在各个行业的重要性，人们一直在努力寻找更合适的用于生产过氧化氢酶的细菌来源。芽孢杆菌属是著名的产过氧化氢酶属之一。这是关于一种新的过氧化氢酶高产菌株芽孢杆菌属ASB 107的首次报道。
芽孢杆菌属ASB 107是我们之前的报告中从拉姆萨尔的阿贝 - 西亚赫泉分离出来的。细菌生物质先冷冻、解冻，然后分别通过三种不同操作进行裂解：超声处理、裂解缓冲液处理和酶消化。粗提物进行硫酸铵沉淀（饱和度为40%和60%）。分别通过电泳和测量比活性来检查半纯化产物的质量和数量。
芽孢杆菌属ASB 107可以通过冻融阶段随后进行溶菌酶消化来裂解，而不能通过裂解缓冲液和超声处理来裂解。令人惊讶的是，芽孢杆菌属ASB 107粗提物中过氧化氢酶的比活性测量值为195370 U/mg蛋白质，这比其他细菌菌株高得多。该细菌显示出约40小时的相对较长的生长曲线。使用硫酸铵沉淀进行半纯化导致比活性提高到约7×10 U/mg蛋白质，这意味着纯化倍数超过3.6倍。
我们已经证明了芽孢杆菌属ASB 107具有天然的过氧化氢酶高产能力。与其他细菌来源相比，芽孢杆菌属ASB 107产生的过氧化氢酶的产量和纯度在工业应用中显示出巨大潜力，表明该菌株是大规模生产过氧化氢酶以处理含HO废水的良好来源。
