Department of Clinical Biochemistry, Aarhus University Hospital, Denmark.
Thromb Res. 2012 Sep;130(3):420-3. doi: 10.1016/j.thromres.2012.06.017. Epub 2012 Jul 17.
Analyses of platelet aggregation in hirudin whole blood using Multiplate® was validated. Reference intervals for the most commonly used agonists were established, and the association between platelet aggregation, age, gender and haematological values was analysed.
We included 121 healthy individuals to establish reference intervals and six healthy individuals for evaluation of the day-to-day variation. Platelet aggregation was evaluated on hirudin whole blood employing Multiplate® induced by arachidonic acid, ADP, collagen and ristocetin (RISTOlow and RISTOhigh). Measurements of haematological values were performed employing Sysmex K-4500.
We found no association between platelet aggregation and age (p>0.57 for all agonists, except RISTOlow: p=0.05). Platelet aggregation was significantly higher in women compared to men for all agonists (p<0.0003), except RISTOlow (p=0.05). A reference interval is presented as 95% confidence interval suitable for any age and both sex. Day-to-day variation was <11% for all agonists except for RISTOlow. No association was found between platelet aggregation and haematocrit or red blood cell count after adjusting for age and gender except for RISTOhigh. A positive significant association was found between platelet count and platelet aggregation (p<0.04). Finally, a significant positive association was found between platelet aggregation and white blood cell count for all agonists (p<0.05) except RISTOlow and RISTOhigh (p>0.05).
Reference intervals for platelet aggregation in healthy individuals (age: 17 to 66 years) were established in hirudin whole blood measured by Multiplate® employing the most commonly used agonists.
对使用 Multiplate®在水蛭素全血中进行的血小板聚集分析进行了验证。建立了最常用激动剂的参考区间，并分析了血小板聚集与年龄、性别和血液学值之间的关系。
我们纳入了 121 名健康个体来建立参考区间，并纳入了 6 名健康个体来评估日常变异。使用 Multiplate®在水蛭素全血中评估了由花生四烯酸、ADP、胶原和瑞斯托菌素（RISTOlow 和 RISTOhigh）诱导的血小板聚集。使用 Sysmex K-4500 进行血液学值的测量。
我们发现血小板聚集与年龄之间没有关联（所有激动剂的 p>0.57，除了 RISTOlow：p=0.05）。与男性相比，女性的所有激动剂的血小板聚集均显著升高（p<0.0003），除了 RISTOlow（p=0.05）。呈现了适合任何年龄和性别的 95%置信区间的参考区间。除了 RISTOlow，所有激动剂的日内变异均<11%。调整年龄和性别后，除了 RISTOhigh，血小板聚集与血细胞比容或红细胞计数之间没有关联。血小板计数与血小板聚集之间存在显著正相关（p<0.04）。最后，除了 RISTOlow 和 RISTOhigh（p>0.05）外，所有激动剂的血小板聚集与白细胞计数之间均存在显著正相关（p<0.05）。
在使用 Multiplate®在水蛭素全血中测量时，建立了健康个体（年龄：17 至 66 岁）的血小板聚集参考区间，使用了最常用的激动剂。
