Engelbrecht Dewaldt, Coetzer Thérèsa Louise
Plasmodium Molecular Research Unit, Department of Molecular Medicine and Haematology, School of Pathology, University of Witwatersrand and National Health Laboratory Service, Wits Medical School, 7 York Road, Parktown, Johannesburg 2193, South Africa.
Parasitol Int. 2012 Dec;61(4):731-4. doi: 10.1016/j.parint.2012.07.002. Epub 2012 Jul 15.
Hydroethidine has been used as a viability dye in various haemoparasites, including Plasmodium species. We compared flow cytometric quantification by hydroethidine and thiazole orange in P. falciparum. Dead parasites that did not develop or replicate exhibited high levels of DNA fragmentation and abnormal microscopic morphology, but were detected as viable ring-stage parasites by hydroethidine. Hydroethidine quantification was similar to thiazole orange, a DNA-binding dye that stains live and dead parasites. Data obtained cast concerns on hydroethidine as a suitable viability dye in P. falciparum and highlight the necessity of proper gating in flow cytometric studies quantifying parasitaemia.
氢化乙锭已被用作包括疟原虫属在内的各种血液寄生虫的活力染料。我们比较了氢化乙锭和噻唑橙对恶性疟原虫进行流式细胞术定量分析的效果。未发育或未复制的死亡寄生虫表现出高水平的DNA片段化和异常的微观形态，但通过氢化乙锭检测为存活的环状期寄生虫。氢化乙锭定量分析结果与噻唑橙相似，噻唑橙是一种可对活的和死亡的寄生虫进行染色的DNA结合染料。所获得的数据引发了对氢化乙锭作为恶性疟原虫合适活力染料的担忧，并突出了在流式细胞术研究中对疟原虫血症进行定量时正确设门的必要性。
