Jiangsu Key Laboratory of Experimental and Translational Non-Coding RNA Research, School of Medicine, Yangzhou University, Yangzhou 225009, China.
Affiliated Hospital of Yangzhou University, Yangzhou 225000, China.
Molecules. 2022 Jun 29;27(13):4163. doi: 10.3390/molecules27134163.
To verify the size and emergence time of new permeability pathways (NPPs) in malaria parasites, the permeability of the -infected erythrocytes was tested with different particle sizes of nanomaterials by flow cytometry assay. The results confirmed the permeability of the host cell membrane increases with parasite maturation for the stage-development evolution of NPPs, and especially found that a particle size of about 50 nm had higher efficiency. As a kind of the novel nanomaterials, nitrogen-doped carbon dots (NCDs) showed no toxicity, specificity binding ability to the malaria parasites, and could label live elder blood-stage through NPPs, indicating the potential application in cell imaging. NPPs and some nanomaterials such as NCDs deserve more attention and exploration for the elimination and prevention of malaria.
为了验证疟原虫中新的通透性途径（NPPs）的大小和出现时间，通过流式细胞术检测了不同粒径纳米材料对 - 感染红细胞的通透性。结果证实，随着 NPPs 的阶段发育演变，寄生虫成熟后宿主细胞膜的通透性增加，特别是发现粒径约为 50nm 的纳米材料具有更高的效率。作为一种新型纳米材料，氮掺杂碳点（NCDs）没有显示出毒性，对疟原虫具有特异性结合能力，并且可以通过 NPPs 对老年血期寄生虫进行标记，这表明其在细胞成像方面具有潜在的应用。NPPs 和一些纳米材料（如 NCDs）值得更多关注和探索，以消除和预防疟疾。
