Lin Lin, Wang Ping, Zhao Xiao-lan
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 May;43(3):335-9.
To investigate the effect and possible mechanism of curcumin to induce apoptosis in ovarian cancer resistant cell lines COC1/DDP.
COC1/DDP cells were treated with different concentration of curcumin, with or without the combination of chemotherapy drugs cisplatin (DDP) and paclitaxel (PIX) for 48 hours. The growth inhibition rates of COC1/DDP cells were studied by MTT method, and the apoptotic ratios were measured with flow cytometry. The expression of phosphoinositide 3-kinase catalytic subunit (PI3KCA) mRNA was studied by RT-PCR in curcumin treated cells, DDP treated cells and their combination treated cells.
After the treatment of different concentration of curcumin for 48 hours, the growth inhibition rates and the apoptotic rate of COC1/DDP cells were gradually increased accordingly with increasing curcumin concentration. Furthurmore, curcumin in combination with chemotherapy drug obtained higher inhibition rate and apoptosis rate than single chemotherapy drug did (P < 0.05). The expression of PI3KCA mRNA of COC1/DDP cells treated with curcumin combined DDP was much lower than that treated only with DDP (P < 0.05).
Curcumin can increase the apoptotic rate of COC1/DDP cells, so has synergistic effect on with chemotherapy drugs on the induction of cell apoptosis. Its possible mechanism may be related to the down-regulation of PI3KCA.
探讨姜黄素诱导卵巢癌耐药细胞株COC1/DDP凋亡的作用及可能机制。
用不同浓度姜黄素处理COC1/DDP细胞，同时或不同时联合化疗药物顺铂（DDP）和紫杉醇（PIX）处理48小时。采用MTT法研究COC1/DDP细胞的生长抑制率，用流式细胞术检测凋亡率。采用RT-PCR法研究姜黄素处理组、DDP处理组及其联合处理组细胞中磷酸肌醇3激酶催化亚基（PI3KCA）mRNA的表达。
不同浓度姜黄素处理48小时后，COC1/DDP细胞的生长抑制率和凋亡率随姜黄素浓度的增加而逐渐升高。此外，姜黄素与化疗药物联合应用的抑制率和凋亡率高于单一化疗药物（P < 0.05）。姜黄素联合DDP处理的COC1/DDP细胞中PI3KCA mRNA的表达明显低于仅用DDP处理的细胞（P < 0.05）。
姜黄素可提高COC1/DDP细胞的凋亡率，与化疗药物联合应用对诱导细胞凋亡具有协同作用。其可能机制可能与下调PI3KCA有关。
