Anal Chem. 2012 Aug 7;84(15):6317-20. doi: 10.1021/ac301541r. Epub 2012 Jul 18.
Differential ion mobility spectrometry (field asymmetric waveform ion mobility spectrometry (FAIMS)) is emerging as a broadly useful tool for separation of isomeric modified peptides with post-translational modifications (PTMs) attached to alternative residues. Such separations were anticipated to become more challenging for smaller PTMs and longer peptides. Here, we show that FAIMS can fully resolve localization variants involving a PTM as minuscule as methylation, even for larger peptides in the middle-down range.
差分离子迁移谱（场非对称波形离子迁移谱（FAIMS））作为一种广泛应用的工具，正在崭露头角，可用于分离连接在替代残基上的具有翻译后修饰（PTM）的异构修饰肽。这种分离预计对于更小的 PTM 和更长的肽变得更具挑战性。在这里，我们表明 FAIMS 甚至可以完全解析涉及小至甲基化等 PTM 的定位变体，即使是中下部范围的较大肽。
