海洋放线菌属 Salinispora 中的铁摄取受去铁胺类铁载体控制。
Iron acquisition in the marine actinomycete genus Salinispora is controlled by the desferrioxamine family of siderophores.
机构信息
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California at San Diego, La Jolla, CA 92093, USA.
出版信息
FEMS Microbiol Lett. 2012 Oct;335(2):95-103. doi: 10.1111/j.1574-6968.2012.02641.x. Epub 2012 Aug 14.
Abstract
Many bacteria produce siderophores for sequestration of growth-essential iron. Analysis of the Salinispora genomes suggests that these marine actinomycetes support multiple hydroxamate- and phenolate-type siderophore pathways. We isolated and characterized desferrioxamines (DFOs) B and E from all three recognized Salinispora species and linked their biosyntheses in S. tropica CNB-440 and S. arenicola CNS-205 to the des locus through PCR-directed mutagenesis. Gene inactivation of the predicted iron-chelator biosynthetic loci sid2-4 did not abolish siderophore chemistry. Additionally, these pathways could not restore the native growth characteristics of the des mutants in iron-limited media, although differential iron-dependent regulation was observed for the yersiniabactin-like sid2 pathway. Consequently, this study indicates that DFOs are the primary siderophores in laboratory cultures of Salinispora.
摘要
许多细菌产生铁载体来螯合生长必需的铁。对盐单胞菌基因组的分析表明，这些海洋放线菌支持多种羟肟酸和酚盐型铁载体途径。我们从所有三种公认的盐单胞菌中分离和鉴定了去铁胺 (DFO) B 和 E，并通过 PCR 定向诱变将其在 S. tropica CNB-440 和 S. arenicola CNS-205 中的生物合成与 des 基因座联系起来。预测的铁螯合生物合成基因座 sid2-4 的基因失活并没有消除铁载体化学。此外，尽管观察到类似于耶尔森氏菌铁载体的 sid2 途径的差异铁依赖性调节，但这些途径不能恢复缺铁培养基中 des 突变体的天然生长特性。因此，本研究表明 DFO 是盐单胞菌实验室培养物中的主要铁载体。
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