应用 RAPD-PCR 对柱状屈挠杆菌进行 DNA 指纹图谱分析。
DNA fingerprinting of Flavobacterium columnare using RAPD-PCR.
出版信息
Indian J Microbiol. 2010 Oct;50(Suppl 1):10-6. doi: 10.1007/s12088-010-0070-5. Epub 2010 Nov 25.
In the present study, DNA fingerprinting of eight strains of Flavobacterium columnare was done by random amplification of polymorphic DNA (RAPD) fingerprinting method. The strains were collected from Fish Health Management Division, Central Institute of Freshwater Aquaculture, Bhubaneswar, India. A total number of 160 primers were screened for RAPD-PCR, of which 10 primers yielded amplification with all the strains. The molecular weight of amplified bands varied from 0.29-2.63 Kb. The number of bands varied from 1 to 8. Unique band was seen with primer OPY-15 with molecular weight 0.75 Kb that can be used for epidemiological study. Genetic variability was investigated using NTSYS software. Highest genetic similarity was found between MS1 and MS3 followed by MS5 and MS7. Minimum genetic similarity was found between MS2 and MS8. Phylogenetic tree was constructed using UPGMA and neighbor joining methods.
在本研究中，采用随机扩增多态性 DNA（RAPD）指纹分析方法对 8 株柱状屈挠杆菌进行了 DNA 指纹分析。这些菌株均采自印度布巴内斯瓦尔中央淡水水产养殖研究所鱼类健康管理部。对总共 160 个引物进行了 RAPD-PCR 筛选，其中 10 个引物对所有菌株均进行了扩增。扩增片段的分子量从 0.29-2.63 Kb 不等。带的数量从 1 到 8 不等。引物 OPY-15 产生的 0.75 Kb 的扩增条带是独特的，可用于流行病学研究。使用 NTSYS 软件对遗传变异进行了研究。MS1 和 MS3 之间以及 MS5 和 MS7 之间发现的遗传相似性最高。MS2 和 MS8 之间发现的遗传相似性最小。使用 UPGMA 和邻接法构建了系统发育树。
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