Department of Biotechnology & Environmental Sciences, Thapar University, Patiala, 147 004 Punjab India.
Indian J Microbiol. 2010 Oct;50(Suppl 1):34-40. doi: 10.1007/s12088-010-0063-4. Epub 2010 Nov 25.
A case study was undertaken for the treatment of domestic wastewater generated at village of Sanghol, Distt. Fatehgarh Sahib, Punjab (India), using a schematic designed algal and duckweed based stabilization pond system, which is discussed here for winter months only (November to March) as there was no growth of duckweeds and only algae dominated the whole system. A proficient increase in pH and dissolved oxygen was observed after the treatment with reduction in chemical oxygen demand and biochemical oxygen demand by 93% and 79% respectively. Chlorella sp. was the dominating algal species in the stabilization pond water during entire period and was studied for its Zn(2+) and Pb(2+) metal removal efficiency. 60-70% removal of Zn(2+) was observed from culture medium containing 5-20 mg L(-1) Zn(2+), which declined to 42% at 50 mg L(-1). A constant decline in cell number from 538 × 10(5) to 8 × 10(5) cells ml(-1) was observed indicating zinc toxicity to Chlorella. Lead was maximally removed by 66.3% from culture medium containing 1 mg L(-1). The lead removal efficiency was 45 50 % at higher 5 to 20 mg L(-1) of external lead concentrations. The increase in cell number indicated no signs of Pb(2+) toxicity up to 20 mg L(-1). The maximum uptake (q(max)) by live Chlorella biomass for both Zn(2+) and Pb(2+) was 34.4 and 41.8 mg/g respectively.
对印度旁遮普邦法塔赫加尔沙布地区 Sanghol 村的生活污水进行了一项案例研究，采用了一种藻类和浮萍为基础的稳定塘系统设计方案。本文仅讨论冬季（11 月至 3 月）的情况，因为浮萍在冬季没有生长，整个系统只有藻类占主导地位。处理后，pH 值和溶解氧显著增加，化学需氧量和生化需氧量分别降低了 93%和 79%。在整个稳定塘水中，优势藻类为小球藻，研究了其对 Zn(2+)和 Pb(2+)的去除效率。在含 5-20mg/L Zn(2+)的培养基中，Zn(2+)的去除率为 60-70%，在 50mg/L 时降至 42%。细胞数量从 538×10(5)个/ml 降至 8×10(5)个/ml，表明锌对小球藻有毒。在含 1mg/L Pb(2+)的培养基中，Pb(2+)的去除率最大为 66.3%。在更高的 5 至 20mg/L 外部铅浓度下，铅的去除效率为 45-50%。细胞数量的增加表明在 20mg/L 以下没有 Pb(2+)毒性的迹象。活小球藻生物量对 Zn(2+)和 Pb(2+)的最大吸附量（q(max)）分别为 34.4 和 41.8mg/g。
