Rachlin J W, Grosso A
Department of Biological Sciences, Lehman College, City University of New York, West, Bronx 10468-1589.
Arch Environ Contam Toxicol. 1993 Jan;24(1):16-20. doi: 10.1007/BF01061084.
Using the growth response of the green alga Chlorella vulgaris as a model system, the effects of combinations of the environmentally active cations Cd, Co, and Cu were evaluated. The 96-h static EC50 for these cations to C. vulgaris were, respectively, 0.89 microM, 9.0 microM, and 2.8 microM, yielding a toxicity series such that Cd > Cu > Co. The cation combinations of Cd + Cu, and Cu + Co acted synergistically, while Cd + Co, and the tri-metallic combination Cd + Cu + Co resulted in antagonistic interactions. Examination of these toxic combinations at 24, 48, 72, and 96 h indicate that the cellular response is not a uniform one. Failure of energy dispersive X-ray spectrophotometric analysis to demonstrate any intracellular incorporation of these cations (except for a weak cytoplasmic Cu peak at the 8.0 KEV position) suggests that the toxic actions of these cations at EC50 concentrations are exerted at the level of the plasma membrane.
以绿藻普通小球藻的生长反应作为模型系统，评估了环境活性阳离子镉（Cd）、钴（Co）和铜（Cu）组合的影响。这些阳离子对普通小球藻的96小时静态半数有效浓度（EC50）分别为0.89微摩尔、9.0微摩尔和2.8微摩尔，得出毒性顺序为Cd > Cu > Co。Cd + Cu和Cu + Co的阳离子组合表现出协同作用，而Cd + Co以及三金属组合Cd + Cu + Co则产生拮抗相互作用。在24、48、72和96小时对这些毒性组合进行检测表明，细胞反应并非一致。能量色散X射线光谱分析未能证明这些阳离子有任何细胞内摄入（除了在8.0千电子伏特位置有一个微弱的细胞质铜峰），这表明这些阳离子在EC50浓度下的毒性作用是在质膜水平发挥的。
