Choi Jong-Ho, Lee Hyun-Jung, Yang Tae-Hyun, Kim Gi Jin
Department of Biomedical Science, CHA University, Seoul, Korea.
Clin Exp Reprod Med. 2012 Jun;39(2):73-80. doi: 10.5653/cerm.2012.39.2.73. Epub 2012 Jun 30.
This study was undertaken to determine the effect of hypoxia inducible factor (HIF)-1α on the cell death, autophagy, and invasion of trophoblasts.
To understand the effect of HIF-1α, we inhibited HIF-1α using siRNA under normoxia and hypoxia conditions. Invasion assay and zymography were performed to determine changes in the invasion ability of HIF-1α. Western blotting and immunofluorescence were performed to determine some of the signal events involved in apoptosis and autophagy.
There was no difference in cell death through the inhibition of HIF-1α expression by siRNA; however, the expression of LC3 and autophagosome formation increased. On the other hand, autophagy was increased, and the invasive ability of trophoblast cells decreased according to the inhibition of HIF-1α expression by siRNA. These experimental results mean that HIF-1α genes regulate the invasive ability of trophoblasts by increasing autophagy.
This study contributes important data for understanding the mechanism of early pregnancy implantation and the invasive ability of trophoblasts by defining the relationship between the roles of HIF-1α and autophagy.
本研究旨在确定缺氧诱导因子（HIF）-1α对滋养层细胞死亡、自噬和侵袭的影响。
为了解HIF-1α的作用，我们在常氧和缺氧条件下使用小干扰RNA（siRNA）抑制HIF-1α。进行侵袭试验和酶谱分析以确定HIF-1α侵袭能力的变化。进行蛋白质免疫印迹法和免疫荧光法以确定参与细胞凋亡和自噬的一些信号事件。
通过siRNA抑制HIF-1α表达，细胞死亡无差异；然而，微管相关蛋白1轻链3（LC3）的表达和自噬体形成增加。另一方面，根据siRNA对HIF-1α表达的抑制，自噬增加，滋养层细胞的侵袭能力降低。这些实验结果表明，HIF-1α基因通过增加自噬来调节滋养层细胞的侵袭能力。
本研究通过确定HIF-1α和自噬作用之间的关系，为理解早期妊娠着床机制和滋养层细胞的侵袭能力提供了重要数据。
