Endocrinology Department, Jinshan Hospital, Fudan University, Shanghai, China.
Autoimmunity. 2012 Nov;45(7):533-9. doi: 10.3109/08916934.2012.702814. Epub 2012 Aug 17.
Th17 lymphocyte and its relative cytokines have been shown to play an important role in autoimmune thyroid diseases (AITD). The aim of this study was to investigate the association between IL-17A and IL-17F gene polymorphisms and two main types of AITD, Graves' disease (GD) and Hashimoto's thyroiditis (HT). Whole blood specimens and clinical data were collected from 508 AITD patients (326 with GD and 182 with HT) and 224 age- and gender-matched healthy controls, respectively. IL-17A (rs2275913, rs8193037, rs3819025) polymorphism was determined using DNA sequencing method and IL-17F/rs763780 polymorphism was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR -RFLP). The results indicated that the frequencies of IL-17F/rs763780 genotypes in patients with GD and HT differed significantly from their controls (P = 0.013 and P = 0.005, respectively); the G allele frequencies were also significantly higher in the patient groups than the control groups (P = 0.002 and 0.001, respectively). For IL-17A/rs2275913 and rs8193037 SNP, no significant difference was observed in patients with either GD or HT compared to the control groups (P>0.05). Interestingly, for rs3819025, the frequency of A allele was lower in patients with GD than controls (P = 0.011). The frequencies of haplotype AGGG and GGGG in patients with GD and HT were significantly higher than in controls (P = 0.012, P = 0.019, P = 0.017 and P = 0.029, respectively). In conclusion, the results indicate that IL-17F/rs763780 polymorphisms may affect the susceptibility to AITD, and IL-17A/rs3819025 SNP is likely a protective factor to GD in the Chinese population.
辅助性 T 细胞 17（Th17）及其相关细胞因子在自身免疫性甲状腺疾病（AITD）中起着重要作用。本研究旨在探讨白细胞介素 17A（IL-17A）和白细胞介素 17F（IL-17F）基因多态性与两种主要 AITD 格雷夫斯病（GD）和桥本甲状腺炎（HT）之间的关联。分别采集了 508 例 AITD 患者（326 例 GD 和 182 例 HT）和 224 例年龄和性别匹配的健康对照者的全血标本和临床资料。采用 DNA 测序法检测 IL-17A（rs2275913、rs8193037、rs3819025）多态性，聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法检测 IL-17F/rs763780 多态性。结果表明，GD 和 HT 患者 IL-17F/rs763780 基因型频率与对照组比较差异有统计学意义（P=0.013 和 P=0.005）；患者组 G 等位基因频率也明显高于对照组（P=0.002 和 0.001）。对于 IL-17A/rs2275913 和 rs8193037 SNP，GD 或 HT 患者与对照组比较差异无统计学意义（P>0.05）。有趣的是，对于 rs3819025，GD 患者的 A 等位基因频率低于对照组（P=0.011）。GD 和 HT 患者的 AGGG 和 GGGG 单体型频率明显高于对照组（P=0.012、P=0.019、P=0.017 和 P=0.029）。结论：结果表明，IL-17F/rs763780 多态性可能影响 AITD 的易感性，而 IL-17A/rs3819025 SNP 可能是中国人 GD 的保护因素。
