Pediatric Department, Faculty of Medicine, Benha University, Benha, Egypt.
Clinical and Chemical Pathology Department, Faculty of Medicine, Benha University, Benha, Egypt.
Pediatr Res. 2023 Oct;94(4):1496-1502. doi: 10.1038/s41390-022-02288-1. Epub 2022 Sep 6.
Interleukin-17F (IL-17F), one of the cytokines, is crucial in the pathophysiology of juvenile idiopathic arthritis (JIA). Therefore, we aimed to determine the relation between IL17F 7488A/G and IL17F 7383A/G single-nucleotide polymorphisms and JIA susceptibility and to explain their impact on the disease activity.
Genomic DNA of 70 patients with JIA and 70 age and sex-matched controls were extracted and typed for IL17F 7488A/G and IL17F 7383A/G single-nucleotide polymorphisms, using polymerase chain reaction with sequence-specific primers method, and compared between patients and controls.
When compared to AA participants, children with the AG genotype of the IL17F 7488A/G and IL17F 7383A/G polymorphisms showed a substantially greater risk of JIA. Furthermore, children with the G allele were 2.8 folds more likely to have JIA than the A allele for IL17F 7488A/G polymorphism and 3.72 folds for IL17F 7383A/G polymorphism. Children with AG genotype of IL17F 7383A/G polymorphism were far more likely to have high activity JIA.
The G allele of both IL17F 7488A/G and IL17F7383 A/G polymorphisms is associated with increased JIA susceptibility, and JIA at High Disease Activity was more likely to develop in AG subjects of the IL17F 7383 A/G polymorphism.
The relationship between Interleukin-17F 7488A/G and 7383A/G polymorphisms and risk for JIA has not been recognized before. Impact of Interleukin-17F 7488A/G and 7383A/G genotypes on JIA disease activity. The G allele of both IL17F 7488A/G and IL17F7383 A/G polymorphisms are associated with increased JIA susceptibility. AG genotype of Interleukin-17F 7383 A/G polymorphism compared to AA patients, had a higher probability of developing JIA at a High Disease Activity (HDA) level.
白细胞介素-17F（IL-17F）是细胞因子之一，在青少年特发性关节炎（JIA）的病理生理学中起关键作用。因此，我们旨在确定 IL17F 7488A/G 和 IL17F 7383A/G 单核苷酸多态性与 JIA 易感性之间的关系，并解释其对疾病活动的影响。
提取 70 例 JIA 患者和 70 例年龄和性别匹配的对照者的基因组 DNA，采用聚合酶链反应-序列特异性引物法对 IL17F 7488A/G 和 IL17F 7383A/G 单核苷酸多态性进行分型，并比较患者与对照组之间的差异。
与 AA 参与者相比，IL17F 7488A/G 和 IL17F 7383A/G 多态性的 AG 基因型患儿发生 JIA 的风险显著增加。此外，对于 IL17F 7488A/G 多态性，G 等位基因患儿发生 JIA 的风险比 A 等位基因高 2.8 倍，对于 IL17F 7383A/G 多态性，G 等位基因患儿发生 JIA 的风险比 A 等位基因高 3.72 倍。IL17F 7383A/G 多态性的 AG 基因型患儿更易发生高疾病活动度 JIA。
IL17F 7488A/G 和 IL17F7383A/G 多态性的 G 等位基因均与 JIA 易感性增加相关，IL17F 7383A/G 多态性的 AG 受试者更易发生高疾病活动度 JIA。
以前尚未认识到白细胞介素-17F 7488A/G 和 7383A/G 多态性与 JIA 风险之间的关系。白细胞介素-17F 7488A/G 和 7383A/G 基因型对 JIA 疾病活动度的影响。IL17F 7488A/G 和 IL17F7383A/G 多态性的 G 等位基因均与 JIA 易感性增加相关。与 AA 患者相比，IL17F 7383A/G 多态性的 AG 基因型患者发生高疾病活动度（HDA）水平 JIA 的可能性更高。
