Collinge M A, Althaus F R
Institute of Pharmacology and Biochemistry, University of Zurich, Tierspital, Switzerland.
Mol Gen Genet. 1994 Dec 15;245(6):686-93. doi: 10.1007/BF00297275.
The coding sequence for human poly(ADP-ribose) polymerase was expressed inducibly in Saccharomyces cerevisiae from a low-copy-number plasmid vector. Cell free extracts of induced cells had poly(ADP-ribose) polymerase activity when assayed under standard conditions; activity could not be detected in noninduced cell extracts. Induced cells formed poly(ADP-ribose) in vivo, and levels of these polymers increased when cells were treated with the alkylating agent N-methyl-N'-nitro-N- nitrosoguanidine (MNNG). The cytotoxicity of this agent was increased in induced cells, and in vivo labelling with [3H]adenine further decreased their viability. Increased levels of poly(ADP-ribose) found in cells treated with the alkylating agent were not accompanied by lowering of the NAD concentration.
人多聚（ADP - 核糖）聚合酶的编码序列通过低拷贝数质粒载体在酿酒酵母中可诱导表达。在标准条件下测定时，诱导细胞的无细胞提取物具有多聚（ADP - 核糖）聚合酶活性；在未诱导的细胞提取物中未检测到活性。诱导细胞在体内形成多聚（ADP - 核糖），当用烷基化剂N - 甲基 - N'- 硝基 - N - 亚硝基胍（MNNG）处理细胞时，这些聚合物的水平会增加。该试剂在诱导细胞中的细胞毒性增加，并且用[3H]腺嘌呤进行体内标记进一步降低了它们的活力。在用烷基化剂处理的细胞中发现的多聚（ADP - 核糖）水平升高并未伴随着NAD浓度的降低。
