Monash IVF, Clayton, Victoria, Australia.
Fertil Steril. 2012 Oct;98(4):843-8.e1. doi: 10.1016/j.fertnstert.2012.06.036. Epub 2012 Jul 18.
To compare the clinical utility of a supersensitive fluorescent semen analysis (SSSA) procedure published by Cooper et al. (2006) with a conventional World Health Organization (WHO)-based semen analysis technique in males with severe oligozoospermia or azoospermia who are undergoing fertility assessment.
Prospective single-center study.
IVF clinic.
PATIENT(S): Patients attending an infertility clinic for semen analysis.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Presence of spermatozoa in the ejaculate.
RESULT(S): Semen samples from 100 men were analyzed using conventional WHO 4th Edition semen analysis and determined to be either severely oligozoospermic or azoospermic (reported lower limit of detection of 0.1 million sperm/mL). An aliquot of the same unprocessed sample was also analyzed using the SSSA protocol (reported lower limit of detection of approximately 8000 sperm/mL). The SSSA method confirmed the results of conventional semen analysis in 77% of cases. In 22% of cases, sperm were identified only using SSSA. Overall, SSSA was capable of identifying the presence of sperm in significantly more samples than conventional semen analysis.
CONCLUSION(S): The reliable differentiation of extreme oligospermia from azoospermia has profound implications in fertility management. This paper provides the first data comparing sperm detection rates using SSSA or conventional WHO-based approaches in extreme oligozoospermic and azoospermic men in an IVF setting. Results indicate that approximately one in four men classified as azoospermic by conventional semen analysis may actually have sperm present. The improved sensitivity of the SSSA technique may be of significant benefit to patients, particularly in fertility and assisted reproductive technique decision making.
比较库珀等人（2006 年）发表的超敏荧光精液分析（SSSA）程序与传统的世界卫生组织（WHO）基于精液分析技术在接受生育评估的严重少精子症或无精子症男性中的临床实用性。
前瞻性单中心研究。
IVF 诊所。
就诊于不孕不育诊所进行精液分析的患者。
无。
精液中是否存在精子。
使用传统的 WHO 第 4 版精液分析对 100 名男性的精液样本进行了分析，结果被确定为严重少精子症或无精子症（报告的最低检测限为 0.100 万精子/ml）。同一份未经处理的样本的一部分也使用 SSSA 方案进行了分析（报告的最低检测限约为 8000 个精子/ml）。SSSA 方法在 77%的情况下证实了传统精液分析的结果。在 22%的情况下，只有使用 SSSA 才能识别精子。总体而言，SSSA 能够在显著更多的样本中识别精子的存在，而不是传统的精液分析。
极重度少精子症与无精子症的可靠区分对生育管理具有深远意义。本文首次提供了在 IVF 环境中比较使用 SSSA 或传统的 WHO 基于方法检测严重少精子症和无精子症男性精子检测率的数据。结果表明，通过传统精液分析分类为无精子症的男性中，约有四分之一实际上可能存在精子。SSSA 技术的灵敏度提高可能对患者具有重要意义，特别是在生育和辅助生殖技术决策方面。
