Stem Cells Bank, Atena Hospital, Timisoara, Romania.
J Surg Res. 2013 May;181(2):359-68. doi: 10.1016/j.jss.2012.06.059. Epub 2012 Jul 7.
Accumulating evidence shows the potential of bone marrow-derived endothelial colony-forming cells (bmECFCs) as promising tools for vascular repair. However, knowledge about their in vitro expansion, characterization, and functional behavior is still controversial. We demonstrate the in vitro generation of rat bmECFCs and analyze their ability to promote tissue reperfusion in a chronic hind-limb ischemia model.
Either in vitro-generated and characterized autologous bmECFCs or placebo was injected into ischemic hind limbs of Sprague-Dawley rats. Tissue perfusion was quantified by laser Doppler, in perfusion units (PU), at days 0, 15, and 30.
Rat bmECFCs acquire a typical phenotype (CD34(+)VEGFR2(+)CD133(+)CXCR4(+)CD45(-)), culture, and functional behavior (Dil-ac-LDL+) in vitro. Injection of autologous bmECFCs improves tissue perfusion in ischemic hind limbs (183.5 ± 3.29 PU(bmECFCs/day 30)versus 131 ± 3.9 PU(controls/day 30), P < 0.001).
We conclude that rat bmECFCs promote ischemic tissue reperfusion and their proangiogenic properties are a potential mechanism for this effect.
越来越多的证据表明骨髓源性内皮祖细胞（bmECFCs）具有作为血管修复有前途的工具的潜力。然而，关于它们的体外扩增、特征和功能行为的知识仍然存在争议。我们展示了大鼠 bmECFCs 的体外生成，并分析了它们在慢性后肢缺血模型中促进组织再灌注的能力。
将体外生成和鉴定的自体 bmECFCs 或安慰剂注射到 Sprague-Dawley 大鼠的缺血后肢中。通过激光多普勒，以灌注单位（PU）在第 0、15 和 30 天量化组织灌注。
大鼠 bmECFCs 在体外获得典型的表型（CD34+VEGFR2+CD133+CXCR4+CD45-）、培养和功能行为（Dil-ac-LDL+）。自体 bmECFCs 注射可改善缺血后肢的组织灌注（183.5±3.29 PU（bmECFCs/第 30 天）与 131±3.9 PU（对照/第 30 天）相比，P<0.001）。
我们得出结论，大鼠 bmECFCs 促进缺血组织再灌注，其促血管生成特性是这种作用的潜在机制。
