Laboratoire d'Imagerie et de Neurosciences Cognitives, UMR 7237 CNRS, Université de Strasbourg, IFR 37, GDR CNRS 2905, Strasbourg, France.
Neurobiol Aging. 2013 Mar;34(3):716-30. doi: 10.1016/j.neurobiolaging.2012.06.016. Epub 2012 Jul 20.
The retinal degeneration Pde6b(rd1) (rd) mutation can be a major pitfall in behavioral studies using tg2576 mice bred on a B6:SJL genetic background, 1 of the most widely used models of Alzheimer's disease. After a pilot study in wild type mice, performance of 8- and 16-month-old tg2576 mice were assessed in several behavioral tasks with the challenge of selecting 1 or more task(s) showing robust memory deficits on this genetic background. Water maze acquisition was impossible in rd homozygotes, whereas Y-maze alternation, object recognition, and olfactory discrimination were unaffected by both the transgene and the rd mutation. Spatial memory retention of 8- and 16-month-old tg2576 mice, however, was dramatically affected independently of the rd mutation when mice had to recognize a spatial configuration of objects or to perform the Barnes maze. Thus, the latter tasks appear extremely useful to evaluate spatial memory deficits and to test cognitive therapies in tg2576 mice and other mouse models bred on a background susceptible to visual impairment.
视网膜变性 Pde6b(rd1) (rd) 突变可能是使用 B6:SJL 遗传背景培育的 tg2576 小鼠进行行为研究的主要陷阱，B6:SJL 是阿尔茨海默病最广泛使用的模型之一。在对野生型小鼠进行了初步研究之后，对 8 个月和 16 个月大的 tg2576 小鼠进行了几项行为任务的评估，目的是选择 1 项或多项在这种遗传背景下表现出明显记忆缺陷的任务。rd 纯合子的水迷宫获得是不可能的，而 Y 迷宫交替、物体识别和嗅觉辨别不受转基因和 rd 突变的影响。然而，当 8 个月和 16 个月大的 tg2576 小鼠必须识别物体的空间构型或执行 Barnes 迷宫时，它们的空间记忆保留能力却受到 rd 突变的显著影响。因此，后一种任务似乎非常有用，可以评估 tg2576 小鼠和其他在易发生视力障碍的背景下培育的小鼠模型的空间记忆缺陷，并测试认知疗法。
