Parfait Bouvourné, Galba Jean Beppe, Roger Ponka, Hervé Hervé Ngatanko Abaissou, Balbine Kamleu Kwingwa, Guillaume Camdi Woumitna, Simon Desire Guedang Nyayi, Linda Damo Kamda Jorelle, Léa Blondelle Kenko Djoumessie, Germain Sotoing Taiwe
Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon.
Department of Agriculture, Animal Husbandry and By-Products, National Polytechnique School, University of Maroua, P.O. Box 46, Maroua, Cameroon.
Evid Based Complement Alternat Med. 2022 Apr 11;2022:7576132. doi: 10.1155/2022/7576132. eCollection 2022.
L. Delile (. ) is used in traditional medicine for the treatment of memory impairment. This work aims to evaluate the antioxidant and anticholinesterase potential of BA fruit pulp extract on excitotoxicity induced by monosodium glutamate (MSG). MSG was administered 30 minutes after treatment with . aqueous fruit pulp extract (50, 125, 250, and 500 mg/kg) and vitamin C (100 mg/kg) for 30 days. The negative control group received only MSG, while the control group was given distilled water daily. Behavioral tests parameters (using the novel object recognition, Y-maze, and Barnes maze tests), oxidative stress biomarkers (malondialdehyde, superoxide dismutase, and catalase), nitric oxide, and acetylcholinesterase activity and hippocampal architecture were evaluated. Results obtained revealed that different doses of . significantly reversed the deleterious effect of MSG on memory. This was displayed by a significant ( < 0.05) increment in the percentage of spontaneous alternation in the Y-maze test and a significant ( < 0.001) increase in discrimination index in novel object recognition observed with 500 mg/kg extract dose. Moreover, the extract (250 and 500 mg/kg doses) significantly ( < 0.001) increased direct search strategy and significantly decreased ( < 0.01) the time taken to find the target hole in the Barnes maze. A modulation of hyperactivity was observed after administration of all extract doses compared to the negative control group in the open arena. Furthermore, the highest dose of the extract caused a significant ( < 0.001) improvement in antioxidant enzymes activity, associated with a significant ( < 0.001) decrement in nitric oxide and malondialdehyde concentrations and a significant ( < 0.01) decrease in acetylcholinesterase activity. Treatment with the extract also restored normal hippocampal cell architecture. . fruit pulp extract could thus confer neuroprotection through its antioxidant and anticholinesterase potential.
L. Delile（. ）在传统医学中用于治疗记忆障碍。本研究旨在评估BA果肉提取物对谷氨酸钠（MSG）诱导的兴奋性毒性的抗氧化和抗胆碱酯酶潜力。在用. 果肉水提取物（50、125、250和500 mg/kg）和维生素C（100 mg/kg）处理30分钟后30天给予MSG。阴性对照组仅接受MSG，而对照组每天给予蒸馏水。评估行为测试参数（使用新物体识别、Y迷宫和巴恩斯迷宫测试）、氧化应激生物标志物（丙二醛、超氧化物歧化酶和过氧化氢酶）、一氧化氮、乙酰胆碱酯酶活性和海马结构。获得的结果表明，不同剂量的. 显著逆转了MSG对记忆的有害影响。这表现为在Y迷宫测试中自发交替百分比显著（<0.05）增加，以及在500 mg/kg提取物剂量下观察到的新物体识别中辨别指数显著（<0.001）增加。此外，提取物（250和500 mg/kg剂量）显著（<0.001）增加直接搜索策略，并显著减少（<0.01）在巴恩斯迷宫中找到目标洞所需的时间。与开放场地中的阴性对照组相比，在给予所有提取物剂量后观察到多动的调节。此外，提取物的最高剂量导致抗氧化酶活性显著（<0.001）改善，与一氧化氮和丙二醛浓度显著（<0.001）降低以及乙酰胆碱酯酶活性显著（<0.01）降低相关。用提取物治疗还恢复了正常的海马细胞结构。因此，. 果肉提取物可通过其抗氧化和抗胆碱酯酶潜力赋予神经保护作用。
