MedImmune Ltd., Granta Park, Cambridge CB21 6GH, UK.
Neurosci Lett. 2012 Aug 30;524(2):107-10. doi: 10.1016/j.neulet.2012.07.017. Epub 2012 Jul 20.
Animal models are an integral part of pain research. However, current models tend to rely on evoked responses and there is a belief that non-evoked responses may be a more relevant behavioural readout as the animal responds in a more natural manner. Here, dynamic weight bearing (DWB), a novel method for assessing mechanical hypersensitivity, was evaluated using the Freund's Complete Adjuvant (FCA) model of inflammatory pain in mice. DWB enables the measurement of weight placed through all four paws of a freely moving animal. The data obtained from DWB was compared with data acquired using the standard static weight bearing (incapacitance) test. In both tests reversal of FCA induced mechanical hypersensitivity was investigated using the selective COX2 inhibitor celecoxib. Mice treated with FCA placed less weight through the ipsilateral hindpaw compared to vehicle controls. This reduction was reversed by celecoxib (30mg/kg p.o.) in the dynamic and static weight bearing tests. The data presented here suggests that dynamic weight bearing may provide a novel end point for the development of new analgesics.
动物模型是疼痛研究的一个组成部分。然而，目前的模型往往依赖于诱发反应，人们相信非诱发反应可能是一种更相关的行为读数，因为动物以更自然的方式做出反应。在这里，动态负重（DWB）是一种评估机械性超敏反应的新方法，该方法用于评估弗氏完全佐剂（FCA）诱导的炎性疼痛模型中小鼠的机械性超敏反应。DWB 能够测量自由移动动物的所有四个爪子承受的重量。将 DWB 获得的数据与使用标准静态负重（电容）测试获得的数据进行比较。在这两种测试中，均使用选择性 COX2 抑制剂塞来昔布来研究 FCA 诱导的机械性超敏反应的逆转。与载体对照组相比，接受 FCA 治疗的小鼠对同侧后爪的负重减少。在动态和静态负重测试中，塞来昔布（30mg/kg po）可逆转这种减少。这里提出的数据表明，动态负重可能为新型镇痛药的开发提供新的终点。
