Department of Orthopaedic Surgery, Kochi Medical School, Kochi University, 185-1 Oko-cho Kohasu, Nankoku, 783-8505, Japan.
Sci Rep. 2023 Feb 20;13(1):2926. doi: 10.1038/s41598-023-29802-7.
This study aimed to investigate the additional effect of ovariectomy-induced osteoporosis (OP) on the pathology of knee osteoarthritis (OA) in a rat meniscectomized model, particularly focusing on subchondral bone changes and pain behaviour. Rats were divided into four groups, sham, OP, OA, OP plus OA, and assessed for histology, osteoclast activity, subchondral bone microstructure, and pain-related behaviour. Rats with OP plus OA had significantly increased calcified cartilage and subchondral bone damage scores, increased densities of subchondral osteoclasts in the weight-bearing area, and more porous subchondral trabecular bone compared with rats with OA. Loss of tidemark integrity was observed most frequently in rats with OP plus OA. The density of subchondral osteoclasts correlated with the calcified cartilage and subchondral bone damage score in rats with OA (OA and OP plus OA). No significant differences in the receptor activator of nuclear factor-kappa B ligand (RANKL)/osteoprotegerin (OPG) expression ratio in subchondral bone and pain-related behavioural tests were observed between rats with OA and rats with OP plus OA. In rats with OA, coexisting OP potentially aggravated OA pathology mainly in calcified cartilage and subchondral trabecular bone by increasing subchondral osteoclast activity.
本研究旨在探讨去卵巢诱导的骨质疏松症 (OP) 对大鼠半月板切除模型膝骨关节炎 (OA) 病理的附加影响，特别是关注软骨下骨变化和疼痛行为。将大鼠分为假手术组、OP 组、OA 组和 OP+OA 组，并进行组织学、破骨细胞活性、软骨下骨微观结构和疼痛相关行为评估。与 OA 组大鼠相比，OP+OA 组大鼠的钙化软骨和软骨下骨损伤评分显著增加，承重区软骨下破骨细胞密度增加，软骨下骨小梁更疏松多孔。在 OP+OA 组大鼠中，观察到最多的潮线完整性丧失。OA 组大鼠中，软骨下破骨细胞密度与钙化软骨和软骨下骨损伤评分呈正相关（OA 组和 OP+OA 组）。OA 组和 OP+OA 组大鼠的软骨下骨核因子-κB 受体激活剂配体 (RANKL)/骨保护素 (OPG) 表达比值在软骨下骨和疼痛相关行为测试中无显著差异。在 OA 大鼠中，共存的 OP 通过增加软骨下破骨细胞活性，可能主要加重了钙化软骨和软骨下骨小梁的 OA 病理。
