高盐应激条件会增加苏云金芽孢杆菌中 pAW63 的转移频率。
High-salt stress conditions increase the pAW63 transfer frequency in Bacillus thuringiensis.
机构信息
Laboratory of Food and Environmental Microbiology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
出版信息
Appl Environ Microbiol. 2012 Oct;78(19):7128-31. doi: 10.1128/AEM.01105-12. Epub 2012 Jul 20.
Abstract
Conjugation experiments with Bacillus thuringiensis and transfer kinetics demonstrated that salt stress has a positive impact on plasmid transfer efficiency. Compared to standard osmotic conditions (0.5% NaCl), plasmid transfer occurred more rapidly, and at higher frequencies (>100-fold), when bacteria were exposed to a high-salt stress (5% NaCl) in liquid brain heart infusion (BHI). Under milder salt conditions (2.5% NaCl), only a 10-fold effect was observed in Luria-Bertani broth and no difference was detected in BHI. These observations are particularly relevant in the scope of potential gene exchanges among members of the Bacillus cereus group, which includes food-borne contaminants and pathogens.
摘要
与苏云金芽孢杆菌的结合实验和转移动力学表明，盐胁迫对质粒转移效率有积极影响。与标准渗透压条件（0.5%NaCl）相比，当细菌在液体脑心浸液（BHI）中暴露于高盐胁迫（5%NaCl）时，质粒转移发生得更快，频率更高（>100 倍）。在较温和的盐条件（2.5%NaCl）下，在 LB 肉汤中仅观察到 10 倍的效果，而在 BHI 中未检测到差异。这些观察结果在包括食源性病原体和病原体在内的蜡样芽孢杆菌组成员之间发生潜在基因交换的范围内尤为重要。
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