Nikolakaki H, Giannakouros T, Georgatsos J G
Laboratory of Biochemistry, School of Chemistry, University of Thessaloniki, Greece.
Biofactors. 1990 Oct;2(4):255-8.
Acetyl-coenzyme A (CoASAc) inhibits the rate of incorporation of amino acid into protein in a cell-free system of mouse liver. The effect is more pronounced when exogenous mRNA (tobacco mosaic virus or globin mRNA) rather than endogenous messages are used. Micromolar concentrations of the cofactor block initiation, while millimolar concentrations cause a more general inhibition of the translation process, that affects, in addition, the elongation step. Inclusion of [1-14C]acetyl-CoA in a protein synthesis reaction mixture results in a very rapid and selective labelling of a protein of 200 kd of the 'pH 5' fraction. The possible involvement of the acetylating event in the regulation of protein synthesis is discussed.
乙酰辅酶A（CoASAc）在小鼠肝脏无细胞体系中抑制氨基酸掺入蛋白质的速率。当使用外源mRNA（烟草花叶病毒或珠蛋白mRNA）而非内源性信使时，这种效应更为明显。微摩尔浓度的辅因子会阻断起始过程，而毫摩尔浓度则会对翻译过程产生更普遍的抑制作用，此外还会影响延伸步骤。在蛋白质合成反应混合物中加入[1-14C]乙酰辅酶A会导致“pH 5”组分中一种200kd的蛋白质迅速且选择性地被标记。本文讨论了乙酰化事件在蛋白质合成调控中的可能作用。
