尺寸排阻色谱法在内在无序蛋白质结构分析中的应用

Size-exclusion chromatography in structural analysis of intrinsically disordered proteins.

作者信息

Uversky Vladimir N

机构信息

Department of Molecular Medicine, College of Medicine, University of South Florida, Tampa, FL, USA.

出版信息

Methods Mol Biol. 2012;896:179-94. doi: 10.1007/978-1-4614-3704-8_11.

DOI:10.1007/978-1-4614-3704-8_11

PMID:22821524

Abstract

Gel-filtration chromatography, also known as size-exclusion chromatography (SEC) or gel-permeation chromatography, is a useful tool for structural and conformational analyses of intrinsically disordered proteins (IDPs). SEC can be utilized for the estimation of the hydrodynamic dimensions of a given IDP, for evaluation of the association state, for the analysis of IDP interactions with binding partners, and for the induced folding studies. It also can be used to physically separate IDP conformers based on their hydrodynamic dimensions, thus providing a unique possibility for the independent analysis of their physicochemical properties.

摘要

凝胶过滤色谱法，也称为尺寸排阻色谱法（SEC）或凝胶渗透色谱法，是用于内在无序蛋白质（IDP）结构和构象分析的有用工具。SEC可用于估计给定IDP的流体动力学尺寸、评估缔合状态、分析IDP与结合伴侣的相互作用以及诱导折叠研究。它还可用于根据流体动力学尺寸对IDP构象体进行物理分离，从而为独立分析其物理化学性质提供独特的可能性。