Department of Biotechnology, Panjab University, Chandigarh, India.
Lung. 2012 Oct;190(5):497-504. doi: 10.1007/s00408-012-9407-7. Epub 2012 Jul 21.
β(2)-Adrenergic receptor (β(2)AR), a G-protein coupled receptor, is present on the bronchial smooth muscle cells and results in bronchodilation upon activation. The genetic factors determining β(2)AR expression and function may not only alter the response of an individual to the therapy but also may serve as predictive markers for response to the agonists used in the therapy. The present study aimed at evaluating the role of β(2)AR-16 and β(2)AR-27 gene polymorphisms in asthma.
A case-control study was performed with a total of 824 adult subjects, including 410 asthmatics and 414 healthy controls from regions of North India. The β(2)AR-16 and β(2)AR-27 polymorphisms were genotyped by PCR-RFLP.
Statistical analysis for the β(2)AR-16 polymorphism revealed that the mutant Gly16 allele was significantly associated with asthma, with OR = 0.80, 95 % CI = 0.65-0.99, and P = 0.032. The Gly16/Gly16 mutant genotype also confers decreased risk toward asthma, with OR = 0.65, 95 % CI = 0.41-1.02, and P = 0.049. However, the β(2)AR-27 polymorphism was not associated with asthma as it did not reach statistical significance, with OR = 0.86, 95 % CI = 0.69-1.07, and P = 0.163.
The β(2)AR-16 polymorphism confers a decreased risk toward asthma while the β(2)AR-27 polymorphism is not associated with asthma in the studied North Indian population.
β（2）-肾上腺素能受体（β（2）AR）是一种 G 蛋白偶联受体，存在于支气管平滑肌细胞上，激活后可导致支气管扩张。决定β（2）AR 表达和功能的遗传因素不仅可能改变个体对治疗的反应，而且还可能作为治疗中使用的激动剂反应的预测标志物。本研究旨在评估β（2）AR-16 和 β（2）AR-27 基因多态性在哮喘中的作用。
进行了一项病例对照研究，共纳入 824 名成年受试者，包括来自印度北部地区的 410 名哮喘患者和 414 名健康对照者。通过 PCR-RFLP 对 β（2）AR-16 和 β（2）AR-27 多态性进行基因分型。
β（2）AR-16 多态性的统计分析显示，突变型 Gly16 等位基因与哮喘显著相关，OR=0.80，95%CI=0.65-0.99，P=0.032。Gly16/Gly16 突变基因型也可降低哮喘的发病风险，OR=0.65，95%CI=0.41-1.02，P=0.049。然而，β（2）AR-27 多态性与哮喘无关，因为它没有达到统计学意义，OR=0.86，95%CI=0.69-1.07，P=0.163。
在研究的印度北部人群中，β（2）AR-16 多态性降低了哮喘的发病风险，而β（2）AR-27 多态性与哮喘无关。
