Johansson U, Hellström S, Stenfors L E, Stigbrand T
Department of Oto-Rhino-Laryngology, University of Umeå, Sweden.
Eur Arch Otorhinolaryngol. 1990;248(2):63-7. doi: 10.1007/BF00240221.
Two otomicroscopically well-defined types of otitis media (OM)--one culture-negative serous (SOM) and the other purulent (POM)--were induced in rats by mechanically obstructing the eustachian tube or by splitting the soft palate. Samples of middle ear effusions (MEE) produced and sera were characterized electrophoretically and immunochemically at 1, 2, 3, 4 and 6 weeks after inducing middle ear fluid. The levels of albumin, transferrin, IgG, IgG1, IgG2, IgM, IgA, and secretory IgA (S-IgA) were determined. All proteins increased 3- to 10-fold in the MEE of the SOM group, whereas the concentrations in the POM group were low. Concentrations of some of the MEE proteins were seen to increase during the course of SOM, while concentrations in the POM group appeared to diminish. The protein content of the MEE in SOM and POM changed dynamically during the observation period. These alterations differed between the two types of OM, reflecting the differing mechanisms that produce the MEE content. These findings indicate that sequential characterization of effusion material in animal OM models may elicit information which might allow staging of this complex disease clinically.
通过机械性阻塞咽鼓管或切开软腭，在大鼠中诱发了两种在耳显微镜下定义明确的中耳炎（OM）类型——一种是培养阴性的浆液性中耳炎（SOM），另一种是化脓性中耳炎（POM）。在诱发中耳积液后的第1、2、3、4和6周，对产生的中耳积液（MEE）样本和血清进行了电泳和免疫化学表征。测定了白蛋白、转铁蛋白、IgG、IgG1、IgG2、IgM、IgA和分泌型IgA（S-IgA）的水平。SOM组MEE中所有蛋白质增加了3至10倍，而POM组中的浓度较低。在SOM病程中，一些MEE蛋白质的浓度会升高，而POM组中的浓度似乎会降低。在观察期内，SOM和POM中MEE的蛋白质含量动态变化。这两种类型的OM之间的这些改变有所不同，反映了产生MEE含量的不同机制。这些发现表明，在动物OM模型中对积液材料进行连续表征可能会获得一些信息，从而有助于临床上对这种复杂疾病进行分期。
