Kim Jeong Hwan, Lee Nam Seob, Jeong Yeong Gil, Lee Je-Hun, Kim Eun Ji, Han Seung Yun
Department of Anatomy, Myunggok Research Institute, Konyang University College of Medicine, Daejeon, Korea.
Anat Cell Biol. 2012 Jun;45(2):103-13. doi: 10.5115/acb.2012.45.2.103. Epub 2012 Jun 30.
Phlorotannins (marine algal polyphenols) have been reported to exhibit beneficial biological activities, serving as both antioxidants and anti-inflammatory agents. Among marine algae, Ecklonia cava, a member of the Laminariaceae, is a very popular food regarded as healthy in Korea and Japan. Recently, benefits afforded by phlorotannins in the treatment of various clinical conditions have been reported, but any therapeutic effects of such materials in the treatment of neurodegenerative diseases such as stroke remain unclear. Also, the mechanisms of action of the algal components remain poorly understood. In the present in vivo study, administration of Ecklonia cava polyphenols (ECP) at 10 mg/kg and 50 mg/kg intraperitoneally (i.p.) significantly decreased infarct size and the extent of brain edema in the rat after induction of transient focal ischemia via middle cerebral artery occlusion (MCAO). Further, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay revealed dose-dependent blockage of neuronal apoptosis upon intravenous ECP treatment. Neurobehavioral tests performed over the 6 days after MCAO revealed a reduction in neurological motor performance in control animals, but administration of ECP (50 mg/kg i.p.) prevented this decline. In vitro, a significant neuroprotective effect of ECP was evident when cell viability was assayed after induction of H(2)O(2)-mediated oxidative stress, upon retinoic acid treatment, in the differentiated neuroblastoma cell line SH-SY5Y. Interestingly, ECP blocked the rise in cytosolic calcium, in a dose-dependent manner, in differentiated SH-SY5Y cells exposed to H(2)O(2). Together, the results suggest that ECP exerts neuroprotective effects in the focally ischemic brain by reducing Ca(2+)-mediated neurotoxicity.
据报道，间苯三酚单宁（海洋藻类多酚）具有有益的生物活性，可作为抗氧化剂和抗炎剂。在海洋藻类中，海带科的成员——海蕴，在韩国和日本是一种非常受欢迎的健康食品。最近，有报道称间苯三酚单宁在治疗各种临床病症方面具有益处，但此类物质在治疗中风等神经退行性疾病方面的任何治疗效果仍不清楚。此外，藻类成分的作用机制仍知之甚少。在本体内研究中，通过大脑中动脉闭塞（MCAO）诱导短暂性局灶性缺血后，以10mg/kg和50mg/kg的剂量腹腔注射（i.p.）海蕴多酚（ECP），可显著减小大鼠的梗死面积并减轻脑水肿程度。此外，末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）试验显示，静脉注射ECP治疗后，神经元凋亡呈剂量依赖性阻断。在MCAO后的6天内进行的神经行为测试显示，对照动物的神经运动功能下降，但注射ECP（50mg/kg i.p.）可防止这种下降。在体外，在分化的神经母细胞瘤细胞系SH-SY5Y中，经视黄酸处理并诱导H(2)O(2)介导的氧化应激后检测细胞活力时，ECP具有显著的神经保护作用。有趣的是，在暴露于H(2)O(2)的分化SH-SY5Y细胞中，ECP以剂量依赖性方式阻断了胞质钙的升高。总之，这些结果表明，ECP通过降低Ca(2+)介导的神经毒性，在局灶性缺血性脑中发挥神经保护作用。
